Zappa U, Reinking-Zappa M, Graf H, Espeland M
School of Dental Medicine, Department of Periodontology, University of Berne, Switzerland.
J Clin Periodontol. 1991 Aug;18(7):508-15. doi: 10.1111/j.1600-051x.1991.tb00082.x.
The purpose of the present study was to assess possible associations between episodic probing attachment loss and cell populations in the supracrestal connective tissue in humans. 10 systemically healthy adult patients with untreated advanced periodontitis were monitored during a period of 10 months. At baseline and every month thereafter, probing attachment levels were measured at 6 sites of every tooth using an electronic pressure sensitive probe and flexible stents. Corresponding contralateral sites were identified where 1 site had lost 2 mm or more attachment within the previous month (P), and the other site had not (C). Supracrestal soft tissue biopsies were taken from these sites, processed and cut into 1 micron sections. Cell populations were identified in superficial and deep connective tissue areas by counting fibroblasts, macrophages, plasma cells, mast cells, granulocytes, lymphocytes, endothelial cells and the total number of inflammatory cells. Analysis of variance assessed differences in cell populations between P- and C-sites. There were statistically significantly higher numbers of fibroblasts in the standard areas of C-sites (p less than 0.0001). In P-sites, the numbers of macrophages, plasma cells, mast cells, lymphocytes and total inflammatory cells were significantly higher as compared to C-sites (p = 0.05-0.0001). There were no differences in cell populations between superficial and deep connective tissue areas within P- and C-sites (p greater than 0.2). Clinically assessed episodes of periodontal disease progression may be associated with site-specific shifts in inflammatory cell populations.
本研究的目的是评估人类龈嵴顶结缔组织中发作性探诊附着丧失与细胞群之间可能存在的关联。对10名患有未经治疗的晚期牙周炎的全身健康成年患者进行了为期10个月的监测。在基线时以及此后的每个月，使用电子压敏探针和柔性支架在每颗牙齿的6个部位测量探诊附着水平。确定相应的对侧部位，其中一个部位在前一个月内附着丧失2mm或更多（P），另一个部位未出现这种情况（C）。从这些部位获取龈嵴顶软组织活检样本，进行处理并切成1微米厚的切片。通过计数成纤维细胞、巨噬细胞、浆细胞、肥大细胞、粒细胞、淋巴细胞、内皮细胞以及炎症细胞总数，确定浅表和深部结缔组织区域中的细胞群。方差分析评估P部位和C部位之间细胞群的差异。C部位标准区域中的成纤维细胞数量在统计学上显著更高（p小于0.0001）。与C部位相比，P部位的巨噬细胞、浆细胞、肥大细胞、淋巴细胞和炎症细胞总数显著更高（p = 0.05 - 0.0001）。P部位和C部位的浅表和深部结缔组织区域之间的细胞群没有差异（p大于0.2）。临床评估的牙周疾病进展发作可能与炎症细胞群的部位特异性变化有关。
