Zappa U, Reinking-Zappa M, Graf H, Case D
Department of Periodontology, School of Dental Medicine, University of Bern, Switzerland.
J Periodontol. 1992 Sep;63(9):748-52. doi: 10.1902/jop.1992.63.9.748.
The purpose of this study was to evaluate the ability of cell populations to differentiate between untreated progressing periodontitis sites (P) and untreated non-progressing sites (NP). Pairs of biopsies were obtained from untreated periodontal patients, one biopsy from a site which had lost probing attachment of 2 mm or more within the previous month, the other biopsy from a non-progressing site. Cell populations were identified on 1 micron sections in a defined connective tissue area at the junctional epithelium. The cell types counted were fibroblasts, mast cells, monocytes/macrophages, polymorphonuclear leukocytes (PMNs), lymphoid cells, plasma cells, endothelial cells, total inflammatory cells, and the total number of cells. The number of fibroblasts, mast cells, monocytes/macrophages, and inflammatory cells, as well as their percentage of the total number of cells, differed significantly between P- and NP-sites. In addition, the actual total counts differed between groups. The difference between groups was more significant for percent fibroblasts than for any other cell type. It appears that cell populations, particularly fibroblast counts, can aid in the histological discrimination between P and NP periodontitis lesions.
本研究的目的是评估细胞群体区分未经治疗的进展期牙周炎部位（P）和未经治疗的非进展期部位（NP）的能力。从未经治疗的牙周病患者获取成对的活检样本，一份活检样本取自前一个月内探诊附着丧失2毫米或更多的部位，另一份活检样本取自非进展期部位。在结合上皮处的特定结缔组织区域的1微米切片上识别细胞群体。计数的细胞类型有成纤维细胞、肥大细胞、单核细胞/巨噬细胞、多形核白细胞（PMN）、淋巴细胞、浆细胞、内皮细胞、总炎症细胞以及细胞总数。P部位和NP部位之间，成纤维细胞、肥大细胞、单核细胞/巨噬细胞和炎症细胞的数量及其占细胞总数的百分比存在显著差异。此外，各组的实际细胞总数也有所不同。成纤维细胞百分比在组间的差异比其他任何细胞类型都更显著。看来细胞群体，尤其是成纤维细胞计数，有助于对P和NP牙周炎病变进行组织学鉴别。
