Oikawa Daichi, Tsuyama Shoichiro, Akimoto Yoriko, Mizobe Yurika, Furuse Mitsuhiro
Laboratory of Advanced Animal and Marine Bioresources, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
Br J Nutr. 2009 May;101(10):1558-63. doi: 10.1017/S0007114508091253. Epub 2008 Oct 24.
Conjugated linoleic acid (CLA) has anti-obesity effects, but induces fatty liver in mice. The present study investigated whether co-administration of arachidonic acid (ARA) attenuates fat accumulation in the mouse liver induced by CLA. Male mice (8 weeks old) were given diets with either no addition of dietary fat (control), 3 % linoleic acid (LA), 3 % CLA, 3 % CLA+1 % ARA, or 3 % CLA+2 % ARA for 4 weeks. The perirenal fat weight in ARA-treated groups decreased similarly as with CLA alone, when compared to control or LA. Plasma TAG concentration was significantly higher in the CLA group than in either CLA+ARA group, while plasma cholesterol and NEFA concentrations did not vary among the groups. In contrast to visceral fat, liver weight was significantly higher in the CLA group than in the control or LA groups, and the effects of CLA were attenuated by ARA. TAG and cholesterol were markedly accumulated in the liver with dietary CLA, whereas co-administration with ARA, at either concentration, suppressed CLA-induced lipid accumulation. Liver PGE(2) was enhanced by a combination of CLA and ARA when compared with CLA alone, but PGE(1) level was not significantly different among groups. In conclusion, fatty liver induced by CLA was attenuated by co-administration with ARA, furthermore, a combination of these fatty acids maintained the anti-obese effect of CLA.
共轭亚油酸（CLA）具有抗肥胖作用，但会诱发小鼠脂肪肝。本研究调查了同时给予花生四烯酸（ARA）是否会减轻CLA诱导的小鼠肝脏脂肪堆积。给8周龄雄性小鼠喂食不含膳食脂肪的饮食（对照）、3%亚油酸（LA）、3%CLA、3%CLA + 1%ARA或3%CLA + 2%ARA的饮食，持续4周。与对照或LA组相比，ARA处理组的肾周脂肪重量与单独使用CLA时相似地减少。CLA组的血浆甘油三酯（TAG）浓度显著高于CLA + ARA组中的任何一组，而各组间血浆胆固醇和非酯化脂肪酸（NEFA）浓度没有变化。与内脏脂肪相反，CLA组的肝脏重量显著高于对照组或LA组，并且ARA减弱了CLA的作用。膳食CLA会使肝脏中TAG和胆固醇明显蓄积，而同时给予任何一种浓度的ARA均可抑制CLA诱导的脂质蓄积。与单独使用CLA相比，CLA和ARA联合使用可提高肝脏前列腺素E2（PGE2）水平，但各组间前列腺素E1（PGE1）水平无显著差异。总之，同时给予ARA可减轻CLA诱导的脂肪肝，此外，这些脂肪酸联合使用可维持CLA的抗肥胖作用。
