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转录终止分析的体外方法。

In vitro approaches to analysis of transcription termination.

作者信息

Artsimovitch Irina, Henkin Tina M

机构信息

Department of Microbiology and Center for RNA Biology, The Ohio State University, 484 W. 12th Avenue, Columbus, OH 43210, USA.

出版信息

Methods. 2009 Jan;47(1):37-43. doi: 10.1016/j.ymeth.2008.10.006. Epub 2008 Oct 21.

DOI:10.1016/j.ymeth.2008.10.006
PMID:18948199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2652710/
Abstract

Transcription termination is an important event in the transcription cycle that has been exploited in a variety of genetic regulatory mechanisms. Analysis of transcription termination is greatly facilitated by in vitro approaches. We describe a basic protocol for analysis of transcription termination in vitro, and include descriptions of parameters that can be modified for specific types of experimental questions.

摘要

转录终止是转录周期中的一个重要事件，已被多种基因调控机制所利用。体外方法极大地促进了转录终止的分析。我们描述了一种体外分析转录终止的基本方案，并包括了可针对特定类型实验问题进行修改的参数说明。

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