Lew J F, Moe C L, Monroe S S, Allen J R, Harrison B M, Forrester B D, Stine S E, Woods P A, Hierholzer J C, Herrmann J E
Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, GA 30333.
J Infect Dis. 1991 Oct;164(4):673-8. doi: 10.1093/infdis/164.4.673.
The relative importance of astrovirus and adenoviruses as etiologic agents of diarrhea among children in day care was examined. Stool specimens from this prospective study were screened for both astrovirus and adenovirus hexon with two new indirect double-antibody assays and for enteric adenoviruses with an EIA specific for serotypes 40 and 41. Astrovirus was detected in a significantly greater percentage of children with diarrhea (4%, 21/524) than of those without (less than 1%, 1/138) (P less than .05); however, no difference between such such children with adenovirus infections was found (8%, 43/565, and 8%, 10/129, respectively). Overall, 30% (13/43) of all adenovirus hexon-positive specimens were enteric serotypes, and by extrapolation, enteric adenoviruses were identified in an equal percentage of children (2%) with and without diarrhea. This study documents the presence of astrovirus and enteric adenoviruses among children in day care in the United States, associates astrovirus with diarrhea in this setting, and suggests that viral agents may be the most common enteric pathogens among children with diarrhea in day care.
研究了星状病毒和腺病毒作为日托中心儿童腹泻病原体的相对重要性。在这项前瞻性研究中，采用两种新的间接双抗体检测法对粪便标本进行星状病毒和腺病毒六邻体筛查，并采用针对40型和41型血清型的酶免疫分析法检测肠道腺病毒。腹泻儿童中检测到星状病毒的比例（4%，21/524）显著高于未腹泻儿童（不到1%，1/138）（P<0.05）；然而，在腺病毒感染的此类儿童之间未发现差异（分别为8%，43/565和8%，10/129）。总体而言，所有腺病毒六邻体阳性标本中有30%（13/43）为肠道血清型，据此推断，腹泻儿童和未腹泻儿童中肠道腺病毒的检出率相同（2%）。本研究证明了美国日托中心儿童中存在星状病毒和肠道腺病毒，表明在这种情况下星状病毒与腹泻有关，并提示病毒病原体可能是日托中心腹泻儿童中最常见的肠道病原体。
