Ma Cuiqing, Wang Ailong, Qin Jiayang, Li Lixiang, Ai Xulu, Jiang Tianyi, Tang Hongzhi, Xu Ping
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
Appl Microbiol Biotechnol. 2009 Feb;82(1):49-57. doi: 10.1007/s00253-008-1732-7. Epub 2008 Oct 24.
Enhanced 2,3-butanediol (BD) production was carried out by Klebsiella pneumoniae SDM. The nutritional requirements for BD production by K. pneumoniae SDM were optimized statistically in shake flask fermentations. Corn steep liquor powder and (NH(4))(2)HPO(4) were identified as the most significant factors by the two-level Plackett-Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal levels. The optimal medium was used to perform fed-batch fermentations with K. pneumoniae SDM. BD production was then studied in a 5-l bioreactor applying different fed-batch strategies, including pulse fed batch, constant feed rate fed batch, constant residual glucose concentration fed batch, and exponential fed batch. The maximum BD concentration of 150 g/l at 38 h with a diol productivity of 4.21 g/l h was obtained by the constant residual glucose concentration feeding strategy. To the best of our knowledge, these results were new records on BD fermentation.
肺炎克雷伯菌SDM实现了2,3-丁二醇（BD）产量的提高。在摇瓶发酵中对肺炎克雷伯菌SDM生产BD的营养需求进行了统计学优化。通过二级Plackett-Burman设计确定玉米浆干粉和磷酸氢二铵（(NH(4))(2)HPO(4)）为最显著因素。采用最速上升实验逼近这两个因素的最佳区域，并采用中心复合设计确定其最佳水平。使用优化后的培养基对肺炎克雷伯菌SDM进行补料分批发酵。然后在5升生物反应器中研究了肺炎克雷伯菌SDM采用不同补料分批策略（包括脉冲补料分批、恒速补料分批、恒定残余葡萄糖浓度补料分批和指数补料分批）时的BD生产情况。通过恒定残余葡萄糖浓度补料策略，在38小时时获得了最高BD浓度150 g/l，二醇生产率为4.21 g/l·h。据我们所知，这些结果是BD发酵的新记录。
