State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
Bioresour Technol. 2010 Mar;101(6):1961-7. doi: 10.1016/j.biortech.2009.10.052. Epub 2009 Nov 20.
The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett-Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by S. marcescens H30 in a 3.7l bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC+BD) productivity of 3.49 g/lh and the yield of 94.67% was obtained.
耻垢分枝杆菌 H30 利用蔗糖生产 2,3-丁二醇（2,3-BD）的研究。通过 Plackett-Burman 设计（PB）和响应面法（RSM）优化了耻垢分枝杆菌 H30 在摇瓶发酵中生产 2,3-BD 的培养基组成。结果表明，酵母提取物和乙酸钠对 2,3-BD 的生产有显著影响。并通过 RSM 确定了它们的最佳浓度。使用优化后的培养基，在 3.7L 生物反应器中进行耻垢分枝杆菌 H30 的发酵实验。比较了几种补料策略，包括中间补料、指数补料和恒残余蔗糖浓度补料，以提高 2,3-BD 的产量。最终，开发了一种将呼吸商（RQ）控制与恒残余蔗糖浓度分批补料相结合的合适控制策略。使用该策略，获得了 139.92g/L 的最大 2,3-BD 浓度，二醇（AC+BD）产率为 3.49g/Lh，产率为 94.67%。
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