Bohman Sara, King Aileen J F
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
Beta Cell Development and Function Group, Division of Reproduction and Endocrinology, Hodgkin Building 2.4N, Guy's Campus, King's College, London SE1 1UL, UK.
Biochem Biophys Res Commun. 2008 Dec 12;377(2):729-733. doi: 10.1016/j.bbrc.2008.10.059. Epub 2008 Oct 23.
Islet transplantation can reverse hyperglycaemia in Type 1 diabetes patients. One problem in islet transplantation is a loss of beta cell mass as well as blunted glucagon responses from the grafted islets. It has been suggested that alpha cell loss is associated with close contact of the alpha cells with the implantation organ. In the present study we made use of microencapsulation, where transplanted islets are not in direct contact with the host implantation site. After transplantation, the number of glucagon cells stained per microencapsulated islet section was increased whereas the number of insulin cells stained was decreased. DNA content of the islets was reduced, as was insulin content, whereas glucagon content was unchanged. This indicates that cell number in transplanted microencapsulated islets diminishes, which can be accounted for by loss of beta cells. However, in contrast to previous studies using non-encapsulated islets, alpha cell number seems to be maintained.
胰岛移植可逆转1型糖尿病患者的高血糖症。胰岛移植中的一个问题是β细胞数量减少以及移植胰岛的胰高血糖素反应减弱。有人提出，α细胞的丢失与α细胞与植入器官的密切接触有关。在本研究中，我们利用了微囊化技术，即移植的胰岛不与宿主植入部位直接接触。移植后，每个微囊化胰岛切片中染色的胰高血糖素细胞数量增加，而染色的胰岛素细胞数量减少。胰岛的DNA含量降低，胰岛素含量也降低，而胰高血糖素含量不变。这表明移植的微囊化胰岛中的细胞数量减少，这可以用β细胞的丢失来解释。然而，与之前使用未封装胰岛的研究不同，α细胞数量似乎得以维持。
