Grillo C A, Reigosa M A, Lorenzo de Mele M Fernández
INIFTA (Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas), UNLP, CONICET, Argentina.
Mutat Res. 2009 Jan;672(1):45-50. doi: 10.1016/j.mrgentox.2008.09.012. Epub 2008 Oct 4.
The aim of this study was to investigate the cytotoxic and genotoxic effect of copper extracts obtained from metallic copper in Chinese hamster ovary (CHO-K1) cell line using neutral red (NR), sister chromatid exchange (SCE), chromosomal aberrations (CA) and cell-cycle kinetics tests. Cells were cultured in Ham-F10 with different copper-containing extracts obtained after the immersion of copper disks for 1, 2, 3, 9, 12, 24, 48 and 72 h in culture medium. Results from cytotoxicity assay showed an inverted U-shape response evidenced in changes in lysosomal activity and mitotic index. The analysis of CA revealed an increase of abnormal metaphases for copper concentration (cCu) in the 5.67-7.42 mg/L dose-range (p<0.001). In addition, SCE frequencies were higher for treated cells when compared with controls in the 1.56-7.42 mg/L concentration range (p<0.001). The absence of metaphases indicated cytotoxicity for cCu≥10.85 mg/L. Results show that cells close to copper-containing materials releasing copper ions are susceptible to cytotoxic and genotoxic effects.
本研究的目的是使用中性红（NR）、姐妹染色单体交换（SCE）、染色体畸变（CA）和细胞周期动力学测试，研究从金属铜中提取的铜提取物对中国仓鼠卵巢（CHO-K1）细胞系的细胞毒性和遗传毒性作用。将铜盘在培养基中浸泡1、2、3、9、12、24、48和72小时后获得的不同含铜提取物，在Ham-F10培养基中培养细胞。细胞毒性试验结果显示出倒U形反应，表现为溶酶体活性和有丝分裂指数的变化。对染色体畸变的分析显示，在5.67-7.42mg/L剂量范围内，随着铜浓度（cCu）的增加，异常中期细胞增多（p<0.001）。此外，在1.56-7.42mg/L浓度范围内，与对照组相比，处理组细胞的姐妹染色单体交换频率更高（p<0.001）。当cCu≥10.85mg/L时，中期细胞的缺失表明存在细胞毒性。结果表明，靠近释放铜离子的含铜材料的细胞易受细胞毒性和遗传毒性作用的影响。
