过量暴露于金属铜释放的铜离子是否会对哺乳动物细胞产生细胞毒性和遗传毒性作用？
Does over-exposure to copper ions released from metallic copper induce cytotoxic and genotoxic effects on mammalian cells?
机构信息
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA, CCT-CONICET La Plata), Facultad de Ciencias Exactas, Departamento de Química, Universidad Nacional de La Plata, La Plata, Argentina.
出版信息
Contraception. 2010 Apr;81(4):343-9. doi: 10.1016/j.contraception.2009.12.003. Epub 2010 Jan 15.
BACKGROUND
A high dissolution of copper from intrauterine devices (IUDs) occurs during the first days after insertion. This work is focused on the assessment of the possible cyto- and genotoxic effects of different concentrations of copper ions released from metallic copper on mammalian cells in vitro.
STUDY DESIGN
Colorimetric tetrazolium/Trypan blue (TB) tests and Comet assay were used to evaluate potential cytotoxicity and genotoxicity, respectively, in Chinese hamster ovary cells (CHO-K1).
RESULTS
Reduction of mitochondrial activity by copper ions was observed for extracts at >or=7.42 mg/L concentrations, while TB exclusion test for plasma membrane integrity showed significant decrease in cell viability (close to 90%) for 10.85 mg/L concentration. Additionally, copper-induced DNA damage was detected for 5.67-7.42 mg/L concentration range.
CONCLUSION
Our results demonstrate cytotoxic and genotoxic effects of copper ions released from metallic copper on CHO-K1 cells and emphasize the importance of reducing the initial copper dissolution from IUD without affecting the contraceptive action.
背景
宫内节育器（IUD）在插入后的最初几天内会大量释放铜离子。本研究旨在评估由金属铜释放的不同浓度的铜离子对体外哺乳动物细胞可能产生的细胞毒性和遗传毒性。
研究设计
采用比色四唑盐/台盼蓝（TB）试验和彗星试验分别评估中国仓鼠卵巢细胞（CHO-K1）的潜在细胞毒性和遗传毒性。
结果
当提取物浓度>或=7.42 mg/L 时，观察到铜离子对线粒体活性的降低，而用于检测质膜完整性的 TB 排除试验表明，对于 10.85 mg/L 浓度，细胞活力显著下降（接近 90%）。此外，在 5.67-7.42 mg/L 的浓度范围内检测到铜诱导的 DNA 损伤。
结论
我们的研究结果表明，金属铜释放的铜离子对 CHO-K1 细胞具有细胞毒性和遗传毒性作用，强调了在不影响避孕效果的情况下减少宫内节育器初始铜溶解的重要性。
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