Ruff Jack A, Lodge Jennifer K, Baker Lorina G
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1100 S. Grand Boulevard, Saint Louis, MO 63104, USA.
Fungal Genet Biol. 2009 Jan;46(1):9-16. doi: 10.1016/j.fgb.2008.10.003. Epub 2008 Oct 14.
Cryptococcus neoformans is the causative agent of cryptococcal meningoencephalitis, most frequently occurring in immunocompromised individuals. There are three varieties of C. neoformans, var. grubii, var. neoformans, and var. gatti. Worldwide var. grubii is the most prevalent clinical isolate. However, few tools for the study of essential genes in var. grubii exist. Here we describe three endogenous inducible promoters for use in the study of this important opportunistic pathogen. We identified eight potential homologs of S. cerevisiae galactose genes in var. grubii. We found that GAL1, GAL7, and UGE2 were regulated by glucose and galactose and can be used successfully during mating. Our analysis indicated these promoters should prove to be excellent tools for analysis of genes in var. grubii.
新型隐球菌是隐球菌性脑膜脑炎的病原体，最常发生于免疫功能低下的个体。新型隐球菌有三个变种，即格氏变种、新型变种和加蒂变种。在全球范围内，格氏变种是最常见的临床分离株。然而，用于研究格氏变种中必需基因的工具很少。在此，我们描述了三种内源性诱导型启动子，用于研究这种重要的机会性病原体。我们在格氏变种中鉴定出了酿酒酵母半乳糖基因的八个潜在同源物。我们发现GAL1、GAL7和UGE2受葡萄糖和半乳糖调控，并且在交配过程中可以成功使用。我们的分析表明，这些启动子应被证明是分析格氏变种中基因的优秀工具。
