Beopoulos Athanasios, Mrozova Zuzana, Thevenieau France, Le Dall Marie-Thérèse, Hapala Ivan, Papanikolaou Seraphim, Chardot Thierry, Nicaud Jean-Marc
Laboratoire de Microbiologie et Génétique Moléculaire, INRA, UMR1238, CNRS, UMR2585, AgroParisTech, Centre de Biotechnologie Agro-Industrielle, BP 01, 78850 Thiverval-Grignon, France.
Appl Environ Microbiol. 2008 Dec;74(24):7779-89. doi: 10.1128/AEM.01412-08. Epub 2008 Oct 24.
A genomic comparison of Yarrowia lipolytica and Saccharomyces cerevisiae indicates that the metabolism of Y. lipolytica is oriented toward the glycerol pathway. To redirect carbon flux toward lipid synthesis, the GUT2 gene, which codes for the glycerol-3-phosphate dehydrogenase isomer, was deleted in Y. lipolytica in this study. This Delta gut2 mutant strain demonstrated a threefold increase in lipid accumulation compared to the wild-type strain. However, mobilization of lipid reserves occurred after the exit from the exponential phase due to beta-oxidation. Y. lipolytica contains six acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX6 genes, that catalyze the limiting step of peroxisomal beta-oxidation. Additional deletion of the POX1 to POX6 genes in the Delta gut2 strain led to a fourfold increase in lipid content. The lipid composition of all of the strains tested demonstrated high proportions of FFA. The size and number of the lipid bodies in these strains were shown to be dependent on the lipid composition and accumulation ratio.
解脂耶氏酵母和酿酒酵母的基因组比较表明，解脂耶氏酵母的代谢倾向于甘油途径。为了将碳通量重定向至脂质合成，本研究中在解脂耶氏酵母中删除了编码3-磷酸甘油脱氢酶异构体的GUT2基因。该Δgut2突变菌株与野生型菌株相比，脂质积累增加了三倍。然而，由于β-氧化作用，脂质储备的动员在指数期结束后发生。解脂耶氏酵母含有六种酰基辅酶A氧化酶（Aox），由POX1至POX6基因编码，它们催化过氧化物酶体β-氧化的限速步骤。在Δgut2菌株中额外删除POX1至POX6基因导致脂质含量增加了四倍。所有测试菌株的脂质组成均显示出高比例的游离脂肪酸（FFA）。这些菌株中脂质体的大小和数量取决于脂质组成和积累率。
