Pisarev Andrei, Poustelnikova Ekaterina, Samsonova Maria, Reinitz John
Department of Computational Biology, St. Petersburg State Polytechnical University, St. Petersburg 195251, Russia.
Nucleic Acids Res. 2009 Jan;37(Database issue):D560-6. doi: 10.1093/nar/gkn717. Epub 2008 Oct 25.
The datasets on gene expression are the valuable source of information about the functional state of an organism. Recently, we have acquired the large dataset on expression of segmentation genes in the Drosophila blastoderm. To provide efficient access to the data, we have developed the FlyEx database (http://urchin.spbcas.ru/flyex). FlyEx contains 4716 images of 14 segmentation gene expression patterns obtained from 1579 embryos and 9,500,000 quantitative data records. Reference data are available for all segmentation genes in cycles 11-13 and all temporal classes of cycle 14A. FlyEx supports operations on images of gene expression patterns. The database can be used to examine the quality of data, analyze the dynamics of formation of segmentation gene expression domains, as well as to estimate the variability of gene expression patterns. Currently, a user is able to monitor and analyze the dynamics of formation of segmentation gene expression domains over the whole period of segment determination, that amounts to 1.5 h of development. FlyEx supports the data downloads and construction of personal reference datasets, that makes it possible to more effectively use and analyze data.
基因表达数据集是有关生物体功能状态的宝贵信息来源。最近，我们获得了果蝇胚盘阶段分割基因表达的大型数据集。为了提供对这些数据的高效访问，我们开发了FlyEx数据库（http://urchin.spbcas.ru/flyex）。FlyEx包含从1579个胚胎中获得的14种分割基因表达模式的4716张图像以及950万个定量数据记录。提供了第11 - 13周期所有分割基因以及第14A周期所有时间类别的参考数据。FlyEx支持对基因表达模式图像进行操作。该数据库可用于检查数据质量、分析分割基因表达域的形成动态，以及估计基因表达模式的变异性。目前，用户能够监测和分析在整个体节确定期间（相当于1.5小时的发育时间）分割基因表达域的形成动态。FlyEx支持数据下载和个人参考数据集的构建，这使得更有效地使用和分析数据成为可能。
