Li Nan, Fan Hong, Chen Hui-li, Ye Yuan-xin, Lu Xiao-jun, Xie Yi, Ying Bin-wu
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhonghua Yi Xue Za Zhi. 2008 May 27;88(20):1380-3.
To investigate the genetic features of the community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) prevalent in West China.
Three CA-MRSA isolates obtained in Chengdu, Sichuan, underwent SCCmec (Staphylococcal Cassette Chromosome mec) multi-PCR, Staphylococcal protein A (spa) typing and multi-locus sequence typing (MLST) method, and their Panton-Valentine leucocidin (PVL) gene was also detected.
All 3 CA-MRSA isolates were positive of mecA gene (147 bp), and the other PCR product of 750 bp was confirmed to be type IVa SCCmec. Spa typing showed that the MRSA strains s29635 and s35301 were typed as t437, and the strain s19165 was typed as t008. MLST showed that the MRSA strains s29635 and s35301 were typed as ST59, and the strain s19165 was typed as ST8. The strains s19165 and s35301 were all positive for PVL gene, and the strain s29635 was negative for PVL gene.
CA-MRSA clones ST8-t008 and ST59-t437 have been isolated in West China.
研究中国西部社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）的基因特征。
对从四川成都分离得到的3株CA-MRSA菌株进行葡萄球菌盒式染色体mec（SCCmec）多重聚合酶链反应（PCR）、葡萄球菌蛋白A（spa）分型及多位点序列分型（MLST），并检测其杀白细胞素（PVL）基因。
3株CA-MRSA菌株的mecA基因（147 bp）均为阳性，另一条750 bp的PCR产物被确认为IVa型SCCmec。spa分型显示，MRSA菌株s29635和s35301为t437型，菌株s19165为t008型。MLST显示，MRSA菌株s29635和s35301为ST59型，菌株s19165为ST8型。菌株s19165和s35301的PVL基因均为阳性，菌株s29635的PVL基因阴性。
在中国西部已分离出CA-MRSA克隆ST8-t008和ST59-t437。
