Geng Wenjing, Yang Yonghong, Wu Dejing, Huang Guoying, Wang Chuanqing, Deng Li, Zheng Yuejie, Fu Zhou, Li Changcong, Shang Yunxiao, Zhao Changan, Yu Sangjie, Shen Xuzhuang
Beijing Children's Hospital Affiliated with the Capital Medical University, Beijing, China.
FEMS Immunol Med Microbiol. 2010 Apr;58(3):356-62. doi: 10.1111/j.1574-695X.2010.00648.x. Epub 2010 Jan 28.
The aim of this study was to investigate the molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from Chinese children. Ninety-nine isolates were collected from eight hospitals, and analyzed by multilocus sequence typing, staphylococcal chromosomal cassette mec (SCCmec) type, and spa typing. The Panton-Valentine leukocidin (PVL) gene was also detected. Overall, 14 sequence types (STs) were obtained, and ST59 (58.6%) was found to be the most prevalent, followed by ST1 (8%) and ST338 (8%). We also first registered the new ST1409. SCCmec type IV was the most predominant type at 67.7%, followed by SCCmec type V at 32.3%. SCCmec subtypes IVa, IVc, and IVg were found among the SCCmec type IV strains. Twenty-one spa types were also identified. Four new spa types were found by synchronization with the Ridom SpaServer and referring to the website (http://www.SeqNet.org). ST59-MRSA-IVa with t437 accounted for 40.4% of occurrences, making it the most prevalent clone. The prevalence of PVL genes was 58.6%, and multidrug resistance was observed in 95% of all isolates. This result indicates that CA-MRSA isolates in Chinese children are largely associated with the ST59-MRSA-IV clone, and that the predominant clones of CA-MRSA are spread all over the country.
本研究旨在调查中国儿童社区获得性耐甲氧西林金黄色葡萄球菌（CA-MRSA）分离株的分子特征。从八家医院收集了99株分离株，并通过多位点序列分型、葡萄球菌染色体盒式mec（SCCmec）分型和spa分型进行分析。还检测了杀白细胞素（PVL）基因。总体而言，共获得14种序列类型（STs），其中ST59（58.6%）最为常见，其次是ST1（8%）和ST338（8%）。我们还首次注册了新的ST1409。SCCmec IV型最为 predominant，占67.7%，其次是SCCmec V型，占32.3%。在SCCmec IV型菌株中发现了SCCmec亚型IVa、IVc和IVg。还鉴定出21种spa类型。通过与Ridom SpaServer同步并参考网站（http://www.SeqNet.org）发现了4种新的spa类型。携带t437的ST59-MRSA-IVa占出现频率的40.4%，是最常见的克隆型。PVL基因的流行率为58.6%，95%的分离株表现出多重耐药性。这一结果表明，中国儿童的CA-MRSA分离株在很大程度上与ST59-MRSA-IV克隆相关，并且CA-MRSA的主要克隆型在全国范围内广泛传播。
