血管紧张素II在蟾蜍皮肤中的水渗透效应：环磷酸腺苷的作用

Hydrosmotic effect of angiotensin II in the toad skin: role of cyclic AMP.

作者信息

Coviello A, Brauckmann E S, de Atenor M S, Apud J A, Causarano J

出版信息

Acta Physiol Lat Am. 1975;25(5):379-86.

Abstract

The mechanism of action of the hydrosmotic response of the isolated skin of the toad Bufo arenarum Hensel to angiotensin II was studied by means of an indirect pharmacological approach. Angiotensin II (2.10(-10) M), vasopressin (2.10(-13) M) and theophylline (10(-4) and 10(-3) M) in subliminal doses produced a significant increase on water permeability when added in different paired combinations. Angiotensin II (2.10(-7) M) and vasopressin (2.10(-8) M) in doses producing significant effects on water permeability increased the response to submaximal doses of epinephrine (10(-6) M) but not to higher doses (10(-5) M). Acid pH (6.4) and prostaglandin E1 (2.10(-7) M) reduced significantly the hydrosmotic response to angiotensin II, but in contrast with the toad bladder, the effect was not completely abolished. Present results support the view that the hydrosmotic effect of angiotensin II in toad skin is mediated by the adenylate cyclase - cyclic AMP system.

摘要

采用间接药理学方法研究了蟾蜍（Bufo arenarum Hensel）离体皮肤对血管紧张素II的渗透反应作用机制。血管紧张素II（2×10⁻¹⁰ M）、血管加压素（2×10⁻¹³ M）和茶碱（10⁻⁴和10⁻³ M）以阈下剂量在不同配对组合中添加时，可使水通透性显著增加。血管紧张素II（2×10⁻⁷ M）和血管加压素（2×10⁻⁸ M）对水通透性产生显著影响的剂量可增强对肾上腺素阈下剂量（10⁻⁶ M）的反应，但对更高剂量（10⁻⁵ M）则无此作用。酸性pH（6.4）和前列腺素E1（2×10⁻⁷ M）显著降低了对血管紧张素II的渗透反应，但与蟾蜍膀胱不同的是，这种作用并未完全消除。目前的结果支持这样一种观点，即血管紧张素II在蟾蜍皮肤中的渗透作用是由腺苷酸环化酶 - 环磷酸腺苷系统介导的。