Bolling Bradley W, Parkin Kirk L
Department of Food Science, University of Wisconsin-Madison, Madison, Wisconsin 53706-1565, USA.
J Agric Food Chem. 2008 Nov 26;56(22):10473-80. doi: 10.1021/jf801541t.
The fractionation of soy flour directed by a cellular bioassay for induction of phase 2 detoxification enzymes was used to identify quinone reductase (QR) inducing agents. A phospholipid-depleted, 80% methanol-partitioned isolate from a crude ethanol extract of soy flour was resolved using normal phase medium-pressure liquid chromatography (MPLC). Early eluting fractions were found to be the most potent QR inducing agents among the separated fractions. Fraction 2 was the most potent, doubling QR at <2 mug/mL. Further fractionation of this isolate led to the identification of several constituents. Fatty acids and sn-1 and sn-2 monoacylglycerols were identified, but were not highly potent QR inducers. Benzofuran-3-carbaldehyde, 4-hydroxybenzaldeyde, 4-ethoxybenzoic acid, 4-ethoxycinnamic acid, benzofuran-2-carboxylic ethyl ester, and ferulic acid ethyl ester (FAEE) were also identified as QR inducing constituents of this fraction. FAEE was the most potent of the identified constituents, doubling QR specific activity at 3.2 muM in the cellular bioassay.
通过细胞生物测定法指导大豆粉分级分离以诱导Ⅱ相解毒酶，用于鉴定醌还原酶（QR）诱导剂。使用正相中压液相色谱（MPLC）对大豆粉粗乙醇提取物中磷脂耗尽的80%甲醇分配分离物进行分离。发现在分离的组分中，早期洗脱的组分是最有效的QR诱导剂。组分2最有效，在浓度<2μg/mL时可使QR活性加倍。对该分离物进一步分级分离导致鉴定出几种成分。鉴定出了脂肪酸以及sn-1和sn-2单酰基甘油，但它们不是高效的QR诱导剂。还鉴定出苯并呋喃-3-甲醛、4-羟基苯甲醛、4-乙氧基苯甲酸、4-乙氧基肉桂酸、苯并呋喃-2-羧酸乙酯和阿魏酸乙酯（FAEE）是该组分的QR诱导成分。FAEE是已鉴定成分中最有效的，在细胞生物测定中，3.2μM时可使QR比活性加倍。
