Joyce Eric F, Tanneti S Nikhila, McKim Kim S
Department of Genetics, Rutgers, the State University of New Jersey, Piscataway, New Jersey 08854-8020, USA.
Genetics. 2009 Jan;181(1):335-40. doi: 10.1534/genetics.108.093104. Epub 2008 Oct 28.
Three Drosophila proteins, ERCC1, MUS312, and MEI-9, function in a complex proposed to resolve double-Holliday-junction intermediates into crossovers during meiosis. We report here the characterization of hold'em (hdm), whose protein product belongs to a single-strand-DNA-binding superfamily of proteins. Mutations in hdm result in reduced meiotic crossover formation and sensitivity to the DNA-damaging agent methyl methanesulfonate. Furthermore, HDM physically interacts with both MEI-9 and ERCC1 in a yeast two-hybrid assay. We conclude that HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.
三种果蝇蛋白，即ERCC1、MUS312和MEI-9，在一个复合物中发挥作用，该复合物被认为在减数分裂过程中将双Holliday连接中间体转化为交叉。我们在此报告“梭哈”（hdm）的特征，其蛋白质产物属于蛋白质的单链DNA结合超家族。hdm中的突变导致减数分裂交叉形成减少以及对DNA损伤剂甲磺酸甲酯的敏感性增加。此外，在酵母双杂交试验中，HDM与MEI-9和ERCC1都发生物理相互作用。我们得出结论，HDM、MEI-9、MUS312和ERCC1形成一个将减数分裂重组中间体转化为交叉的复合物。
