Blonstein A D, Stirnberg P, King P J
Friedrich Miescher-Institut, Basel, Switzerland.
Mol Gen Genet. 1991 Sep;228(3):361-71. doi: 10.1007/BF00260628.
We have isolated nine independent auxin-resistant mutants of Nicotiana plumbaginifolia by culturing M2 seedlings in the presence of indole-3-acetic acid ethyl ester or 1-naphthaleneacetic acid at concentrations which significantly inhibit hypocotyl elongation of the wild type. The mutations were induced by treating seed with ethyl methanesulphonate and were found in the course of screening 10,000 individual M2 families. Auxin resistance was in all cases the result of a mutation at a single, nuclear locus. The dominance relationships of two of the mutants could be defined as recessive or dominant; all other mutants showed partial dominance. In contrast to previously described mutants of Arabidopsis and N. plumbaginifolia, all of the present mutants were specifically resistant to auxin; the mutants were cross-resistant to several auxins, but showed no increased resistance to cytokinin, abscisic acid, ethylene or 1-amino-cyclopropane-1-carboxylic acid. The importance of the choice of the selection criterion for the isolation of specific resistance traits is discussed.
我们通过在吲哚 - 3 - 乙酸乙酯或1 - 萘乙酸存在的情况下培养M2幼苗，分离出了9个独立的烟草抗生长素突变体，其浓度能显著抑制野生型下胚轴伸长。这些突变是通过用甲磺酸乙酯处理种子诱导产生的，在筛选10,000个单独的M2家系过程中被发现。在所有情况下，抗生长素都是单个核基因座发生突变的结果。其中两个突变体的显性关系可定义为隐性或显性；所有其他突变体表现出部分显性。与先前描述的拟南芥和烟草突变体不同，所有当前的突变体对生长素具有特异性抗性；这些突变体对几种生长素具有交叉抗性，但对细胞分裂素、脱落酸、乙烯或1 - 氨基环丙烷 - 1 - 羧酸没有表现出抗性增加。讨论了选择分离特定抗性性状的选择标准的重要性。
