Carcamo J, Buckbinder L, Reinberg D
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8052-6. doi: 10.1073/pnas.88.18.8052.
Highly purified RNA polymerase II was found to be able to weakly recognize the initiator (Inr) present in the adenovirus IVa2 and major late promoters. The association of RNA polymerase II with the Inr was enhanced by the general transcription factors. The Inr was capable of directing the formation of a DNA-protein complex. Transcription competent complexes on the adenovirus major late and IVa2 promoters appear to be formed by alternative pathways mediated through the Inr and/or "TATA" motif. The presence of both motifs, however, is required for efficient transcription utilizing a discrete start site. Complexes formed at either site required transcription factor TFIID, the TATA binding protein. Consistent with this observation, a TFIID requirement was demonstrated for transcription from a mutant adenovirus major late promoter construct lacking a functional TATA motif.
人们发现，高度纯化的RNA聚合酶II能够微弱地识别腺病毒IVa2和主要晚期启动子中存在的起始子（Inr）。通用转录因子可增强RNA聚合酶II与起始子的结合。起始子能够指导DNA-蛋白质复合物的形成。腺病毒主要晚期和IVa2启动子上具有转录活性的复合物似乎是通过起始子和/或“TATA”基序介导的替代途径形成的。然而，要利用离散的起始位点进行高效转录，这两个基序都必须存在。在任一位点形成的复合物都需要转录因子TFIID，即TATA结合蛋白。与这一观察结果一致的是，对于一个缺乏功能性TATA基序的突变腺病毒主要晚期启动子构建体的转录，已证明需要TFIID。
