Askal H F
University of Assiut, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Assiut, Egypt.
Talanta. 1991 Oct;38(10):1155-8. doi: 10.1016/0039-9140(91)80235-r.
Three simple, rapid and sensitive methods for the assay of captopril which is an effective alternative to digitalis were developed. These methods are based on the oxidation reaction in aqueous solution of captopril with either ferric chloride or iodine. The indirect quantitation of the product was carried out at 523, 351 and 620 nm for ferro-bipyridyl, residual iodine and residual iodine-starch complex, respectively. All variables were studied to optimize the reaction conditions. Regression analysis of Beer's plot showed good correlation in a general concentration range of 0.25-25 mug captopril/ml. No interference was observed from hydrochlorothiazide diuretic which was recently introduced in combination with captopril or other common pharmaceutical adjuvants. The validity of the methods was tested by analysing capoten and capozide tablets. Recoveries were 99.1-102.8%.
开发了三种简单、快速且灵敏的卡托普利测定方法，卡托普利是洋地黄的一种有效替代药物。这些方法基于卡托普利在水溶液中与氯化铁或碘的氧化反应。分别在523、351和620nm处对亚铁联吡啶、残留碘和残留碘 - 淀粉络合物进行产物的间接定量。研究了所有变量以优化反应条件。比尔定律曲线的回归分析表明，在卡托普利浓度为0.25 - 25μg/ml的一般范围内具有良好的相关性。未观察到与最近与卡托普利联合使用的氢氯噻嗪利尿剂或其他常见药物佐剂的干扰。通过分析卡托普利片和复方卡托普利片来测试这些方法的有效性。回收率为99.1 - 102.8%。
