Wring S A, Hart J P, Birch B J
Science Department, Bristol Polytechnic, Coldharbour Lane, Frenchay, Bristol, BS16 1QY, U.K.
Talanta. 1991 Nov;38(11):1257-60. doi: 10.1016/0039-9140(91)80102-6.
High-performance liquid chromatography with electrochemical detection by means of a re-polishable graphite-epoxy resin composite electrode, modified with the electrocatalyst cobalt phthalocyanine, has been used for the determination of reduced glutathione (GSH) in 25-mul samples of whole blood. The mobile phase was O.O5M phosphate buffer (pH 2.3) containing 1 mM EDTA, used in conjunction with a Waters muBondapak ODS chromatography column. The use of the electrocatalyst reduced the overpotential for the oxidation of GSH at a carbon electrode by approximately 750 mV, and the applied potential used was +0.5 V vs. Ag/AgCl. The mean recovery of GSH added during the sample pretreatment step was 95%; the assay imprecision was 1-2% for triplicate analyses of the whole blood samples.
采用一种可再抛光的石墨-环氧树脂复合电极，用电催化剂钴酞菁进行修饰，通过高效液相色谱结合电化学检测法，已用于测定25微升全血样品中的还原型谷胱甘肽（GSH）。流动相为含有1 mM乙二胺四乙酸（EDTA）的0.05M磷酸盐缓冲液（pH 2.3），与沃特世μBondapak ODS色谱柱联用。该电催化剂的使用使碳电极上GSH氧化的过电位降低了约750 mV，所施加的电位相对于Ag/AgCl为+0.5 V。样品预处理步骤中添加的GSH的平均回收率为95%；对全血样品进行三次重复分析时，测定的不精密度为1 - 2%。
