Wring S A, Hart J P, Birch B J
Analyst. 1989 Dec;114(12):1571-3. doi: 10.1039/an9891401571.
High-performance liquid chromatography with electrochemical detection (LCEC), incorporating a novel carbon-epoxy resin working electrode modified with cobalt phthalocyanine, has been employed for preliminary studies directed towards the determination of normal circulating levels of reduced glutathione (GSH) in human plasma. The mobile phase consisted of 0.05 M phosphate buffer (pH 3) containing 0.1% m/m ethylenediaminetetraacetic acid (EDTA); the calibration graph was linear in the range 0.24-30.7 ng of GSH injected. The mean recovery of GSH added to a control serum over the physiological concentration range (0.38-3.07 ng ml-1) was 99%; this was achieved following a simple sample pre-treatment method, prior to LCEC, involving chelation of divalent cations with EDTA and subsequent acidification with orthophosphoric acid. Using the LCEC method, the mean circulating level of GSH in plasma, found in three normal subjects, was 2.69 microM, GSH; this indicates that the method might be applicable to the determination of depressed circulating levels of GSH.
采用带有电化学检测的高效液相色谱法（LCEC），该方法采用了一种用钴酞菁修饰的新型碳环氧树脂工作电极，已用于初步研究以测定人血浆中还原型谷胱甘肽（GSH）的正常循环水平。流动相由含有0.1% m/m乙二胺四乙酸（EDTA）的0.05 M磷酸盐缓冲液（pH 3）组成；进样0.24 - 30.7 ng GSH时校准曲线呈线性。在生理浓度范围（0.38 - 3.07 ng ml-1）内添加到对照血清中的GSH平均回收率为99%；这是在LCEC之前采用一种简单的样品预处理方法实现的，该方法包括用EDTA螯合二价阳离子，随后用正磷酸酸化。使用LCEC方法，在三名正常受试者中测得血浆中GSH的平均循环水平为2.69 microM GSH；这表明该方法可能适用于测定GSH降低的循环水平。
