Wickman M, Hedén P, Jurell G
Department of Plastic and Reconstructive Surgery, Karolinska Hospital, Stockholm, Sweden.
Plast Reconstr Surg. 1991 Oct;88(4):650-6. doi: 10.1097/00006534-199110000-00015.
To evaluate circulatory and metabolic changes in pig skin during tissue expansion, the buttock skin of 12 pigs was expanded for 5 weeks. In a second operation, island buttock flaps were elevated bilaterally. Flap temperature, laser Doppler flow (LDF), and fluorescein penetration borders were recorded. Norepinephrine infusions were given twice. After cannulation of the external iliac veins, the total venous outflow from the flaps and metabolic parameters such as glucose and oxygen consumption and lactate production were measured bilaterally. No significant difference in fluorescein staining was found, but laser Doppler flow in the expanded tissue was higher than in nonexpanded skin, whereas the total flap blood flow was not significantly different. A flow reduction was seen in expanded flaps during norepinephrine infusion, whereas nonexpanded flaps showed a slightly increased blood flow. This adrenergic supersensitivity indicates that it is possible that not only surgical nerve section but also tissue expansion can result in sympathetic denervation. No differences in the metabolic parameters were observed.
为评估组织扩张过程中猪皮肤的循环和代谢变化，对12头猪的臀部皮肤进行了5周的扩张。在第二次手术中，双侧掀起岛状臀部皮瓣。记录皮瓣温度、激光多普勒血流（LDF）和荧光素渗透边界。给予去甲肾上腺素输注两次。在髂外静脉插管后，双侧测量皮瓣的总静脉流出量以及葡萄糖、氧消耗和乳酸产生等代谢参数。荧光素染色未发现显著差异，但扩张组织中的激光多普勒血流高于未扩张皮肤，而皮瓣总血流量无显著差异。去甲肾上腺素输注期间，扩张皮瓣出现血流减少，而未扩张皮瓣血流略有增加。这种肾上腺素能超敏反应表明，不仅手术切断神经，组织扩张也可能导致交感神经去神经支配。代谢参数未观察到差异。
