高效液相色谱法同时测定厚朴温中胶囊中7种有效成分

[Simultaneous determination of seven effective component in Houpuwenzhong capsules using high performance liquid chromatography].

作者信息

Ding Xiaoju, Zhao Yunli, Gao Xiaoxia, Tang Qian, Li Lin, Yu Zhiguo

机构信息

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Se Pu. 2009 Jan;27(1):107-10.

PMID:19449552

Abstract

A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of seven effective components in Houpuwenzhong capsules. The separation was performed on a Scienhome C18 column (250 mm x 4.6 mm, 5 microm) with methanol-acetonitrile-0.06% phosphonic acid (38 : 27 : 35, v/v/v) as the mobile phase at a flow rate of 1.0 mL/min and 30 degrees C. The detective wavelength was set at 235 nm. There were good linear relationships between the mass concentrations and the peak areas of alpinetin, glycyrrhizic acid, honokiol, cardamonin, costunolide, dehydrocostus lactone and magnolol in the ranges of 0.885 -17.7, 107 - 2140, 8.85 - 17.7, 1.035 - 20.7, 4.85 - 97, 5.9 - 118 and 17.5 - 350 mg/L, respectively. The recoveries were 96.9% - 101.1%, 96.0% - 100.5%, 100.3% - 100.8%, 97.7% - 101.4%, 100.4% - 102.3%, 96.0% - 102.3% and 96.2% - 100.6%, respectively. This method is rapid, simple and suitable for the quality control of Houpuwenzhong capsules.

摘要

建立了一种高效液相色谱（HPLC）法同时测定厚朴温中胶囊中7种有效成分的含量。采用Scienhome C18色谱柱（250 mm×4.6 mm，5μm），以甲醇-乙腈-0.06%磷酸（38∶27∶35，v/v/v）为流动相，流速为1.0 mL/min，柱温30℃，检测波长为235 nm。山姜素、甘草酸、厚朴酚、小豆蔻明、木香烃内酯、去氢木香内酯和厚朴酚的质量浓度在0.885 - 17.7、107 - 2140、8.85 - 17.7、1.035 - 20.7、4.85 - 97、5.9 - 118和17.5 - 350 mg/L范围内与峰面积均呈良好的线性关系。回收率分别为96.9% - 101.1%、96.0% - 100.5%、100.3% - 100.8%、97.7% - 101.4%、100.4% - 102.3%、96.0% - 102.3%和96.2% - 100.6%。该方法快速、简便，适用于厚朴温中胶囊的质量控制。