Erroz C L, Viñas P, Córdoba M H
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071 Murcia, Spain.
Talanta. 1994 Dec;41(12):2159-64. doi: 10.1016/0039-9140(94)00203-7.
A fluorimetric procedure for the determination of sulfamethoxazole using flow injection analysis is proposed. A two channel system is optimized for the fluorescent reaction with o-phthaldialdehyde and beta-mercaptoethanol. The detection limit is 0.007 mug/ml. No fluorescence is generated by the metabolite N-acetylated sulfamethoxazole. The method is applied to the determination of sulfamethoxazole in pharmaceuticals, urine and bovine serum samples without matrix interference problems.
