Zhang Z, Qin W
Talanta. 1996 Jan;43(1):119-24. doi: 10.1016/0039-9140(95)01710-0.
A novel flow sensor based on chemiluminescence (CL) for the determination of ascorbic acid has been proposed. The analytical reagents, luminol and ferricyanide, were both immobilized on an anion-exchange resin column. The CL signal produced by the reaction between luminol and ferricyanide, which were eluted from the column through sodium phosphate injection, was decreased in the presence of ascorbic acid. The CL emission intensity was linear with ascorbic acid concentration in the range 0.01-0.8 mug ml(-1); the detection limit was 5.5 x 10(-3) mug ml(-1). The whole process, including sampling and washing, could be completed in 1 min with a relative standard deviation of less than 5%. The sensor could be reused more than 100 times and has been applied successfully to the analysis of ascorbic acid in pills and vegetables.
提出了一种基于化学发光（CL）的新型流动传感器用于测定抗坏血酸。分析试剂鲁米诺和铁氰化物均固定在阴离子交换树脂柱上。通过注射磷酸钠从柱中洗脱出来的鲁米诺和铁氰化物之间反应产生的CL信号，在抗坏血酸存在下会降低。CL发射强度与抗坏血酸浓度在0.01 - 0.8μg ml⁻¹范围内呈线性关系；检测限为5.5×10⁻³μg ml⁻¹。整个过程，包括采样和清洗，可在1分钟内完成，相对标准偏差小于5%。该传感器可重复使用100多次，并已成功应用于药丸和蔬菜中抗坏血酸的分析。
