García Falcón S, González Amigo S, Lage Yusty M A, López de Alda Villaizán M J, Simal Lozano J
Talanta. 1996 Apr;43(4):659-64. doi: 10.1016/0039-9140(95)01811-5.
We have developed a simple, rapid, inexpensive method for the determination of benzo[a]pyrene (BP, a known carcinogen) in smoking-flavour agents (water-soluble liquid smoke; WSLS). After purification of the WSLS by a single passage through a Sep Pak C18 Plus cartridge, BP in the hexane eluate was determined by second derivative constant-wavelength synchronous spectrofluorimetry. Method precision (RSD < 6%) and recovery ( approximately 92%) were satisfactory, and the detection and quantification limits (1.05 and 2.28 mug kg(-1) respectively) indicated that the current maximum permissible concentration of BP in smoke flavourings (10 mug kg(-1)) can be monitored by this method.
我们开发了一种简单、快速、廉价的方法，用于测定烟熏调味剂（水溶性液态烟熏剂；WSLS）中的苯并[a]芘（BP，一种已知致癌物）。通过使WSLS单次通过Sep Pak C18 Plus柱进行纯化后，用二阶导数恒波长同步荧光光谱法测定己烷洗脱液中的BP。方法精密度（相对标准偏差<6%）和回收率（约92%）令人满意，检测限和定量限（分别为1.05和2.28μg kg⁻¹）表明，该方法可监测烟熏香料中BP的当前最大允许浓度（10μg kg⁻¹）。
