Sastry C S, Rao S G, Y Naidu P, Srinivas K R
Foods and Drugs Laboratories, Department of Organic Chemistry, Foods, Drugs and Water, Andhra University, Visakhapatnam 530 003, India.
Talanta. 1998 Apr;45(6):1227-34. doi: 10.1016/s0039-9140(97)00237-3.
A simple, sensitive and selective method is proposed for the spectrophotometric determination of drugs, viz. ampicillin, penicillin V, amoxycillin, cloxacillin, cefadroxil, ceftezoxime, griseofulvin, streptomycin, nicoumalone and acebutolol HCl, based on their reactivity with iodine. The method involves the addition of excess iodine of known concentration to the drug in the presence of NaOH and the unreacted iodine is determined by the measurement of the decrease in the absorbance of the dye wool fast blue BL (lambda(max)=540 nm) which was found to be the most suitable of several dyes tested. This method was applied for the determination of drug contents in pharmaceutical formulations and enabled the determination of the drugs in microgram quantities (0.8-9.6 mug ml(-1)). No interferences were observed from excipients and the validity of the method was tested against reference methods.
提出了一种简单、灵敏且具有选择性的分光光度法，用于测定药物，即氨苄西林、青霉素V、阿莫西林、氯唑西林、头孢羟氨苄、头孢唑肟、灰黄霉素、链霉素、尼可马隆和盐酸醋丁洛尔，该方法基于它们与碘的反应性。该方法包括在氢氧化钠存在下，向药物中加入已知浓度的过量碘，未反应的碘通过测量染料羊毛固色蓝BL（λmax = 540 nm）吸光度的降低来测定，在测试的几种染料中，该染料被发现是最合适的。该方法用于测定药物制剂中的药物含量，能够测定微克量（0.8 - 9.6 μg ml-1）的药物。未观察到辅料的干扰，并与参考方法对该方法的有效性进行了测试。
