Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova-Izmir, Turkey.
Talanta. 1999 Aug 23;50(1):87-93. doi: 10.1016/s0039-9140(99)00107-1.
A biosensor for the specific determination of l-ascorbic acid in fruit juices and vitamin C tablets was developed using ascorbate oxidase (EC 1.10.3.3) from cucumber (Cucumis sativus L.) in combination with a dissolved oxygen probe. Ascorbate oxidase immobilized with gelatin using glutaraldehyde and fixed on pretreated teflon membrane served as an enzyme electrode. The phosphate buffer (50 mM, pH 7.5) and 35 degrees C were established as providing the optimum conditions. The biosensor response depends linearly on l-ascorbic acid concentration between 5.0x10(-5) and 1.2x10(-3) M with a response time 45 s. The biosensor is stable for more than 2 months, while more than 200 assays were performed. The results obtained for fruit juices and tablets were compared with DCIP (2,6 dichlorophenolindophenol) method.
采用黄瓜（Cucumis sativus L.）中的抗坏血酸氧化酶（EC 1.10.3.3），结合溶解氧探头，研制出一种用于特定测定果汁和维生素 C 片剂中 L-抗坏血酸的生物传感器。使用戊二醛将明胶固定化的抗坏血酸氧化酶固定在预处理过的特氟龙膜上作为酶电极。磷酸盐缓冲液（50 mM，pH 7.5）和 35°C 被确定为最佳条件。生物传感器的响应与 L-抗坏血酸浓度在 5.0x10(-5) 和 1.2x10(-3) M 之间呈线性关系，响应时间为 45 秒。生物传感器在 2 个月以上的时间内保持稳定，同时进行了 200 多次测定。将所得结果与 DCIP（2,6 二氯酚靛酚）法进行比较。
