Laboratoire d'Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Université Joseph Fourier Grenoble 1, BP 53, 38041 Grenoble Cedex 9, France.
Talanta. 2001 Dec 13;55(5):889-97. doi: 10.1016/s0039-9140(01)00498-2.
Two different biodegradable latex polymers functionalised by hydroxy (1) or gluconamide (2) groups proved to be good immobilisation matrixes for glucose oxidase. The responses of these biosensors to glucose additions were measured by potentiostating the modified electrodes at 0.6 V/SCE in order to oxidise the hydrogen peroxide generated by the enzymatic oxidation of glucose in the presence of oxygen. The response of such electrodes was evaluated as a function of film thickness, pH and temperature. Rotating disk electrode experiments showed the influence of the enzyme on the structure of both latex films, namely a marked improvement in matrix permeability. The high permeability of the latex 1 based enzyme sensor (bilayer, P(m)=8.10x10(-4) cm s(-1)) resulted in a high dynamic range. Furthermore, the activation energy for a latex 1 sensor was determined to be 44.55 and 18.03 kJ mol(-1), respectively depending on the conformation of the enzyme.
两种不同的可生物降解乳胶聚合物通过羟（1）或葡糖酰胺（2）基团进行了功能化，被证明是葡萄糖氧化酶的良好固定化基质。通过在 0.6 V/SCE 下对修饰电极进行电势测定，以氧化在氧气存在下葡萄糖的酶氧化产生的过氧化氢，测量了这些生物传感器对葡萄糖添加的响应。将这些电极的响应作为膜厚度、pH 和温度的函数进行了评估。旋转圆盘电极实验表明了酶对两种乳胶膜结构的影响，即基质渗透性的显著改善。基于乳胶 1 的酶传感器（双层，P(m)=8.10x10(-4) cm s(-1))具有高渗透性，因此具有宽动态范围。此外，取决于酶的构象，乳胶 1 传感器的活化能分别确定为 44.55 和 18.03 kJ mol(-1)。
