Pan Dawei, Chen Jinhua, Nie Lihua, Tao Wenyan, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry, and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
Anal Biochem. 2004 Jan 1;324(1):115-22. doi: 10.1016/j.ab.2003.09.029.
Prussian blue (PB), as a good catalyst for the reduction of hydrogen peroxide, has been combined with nonconducting poly(o-aminophenol) (POAP) film to assemble glucose biosensor. Compared with PB-modified enzymatic biosensor, the biosensor based on glucose oxidase immobilized in POAP film at PB-modified electrode shows much improved stability (78% remains after 30 days) in neutral medium. Additionally, the biosensor, at an applied potential of 0.0 V, exhibits other good characteristics, such as relative low detection limit (0.01 mM), short response time (within 5s), large current density (0.28 mA/cm2), high sensitivity (24 mAM(-1)cm(-2)), and good antiinterferent ability. The apparent activation energy of enzyme-catalyzed reaction and apparent Michaelis-Menten constant are 34.2 KJmol(-1) and 10.5 mM, respectively. In addition, effects of temperature, applied potential used in the determination, pH value of the detection solution, and electroactive interferents on the amperometric response of the sensor were investigated and are discussed.
普鲁士蓝（PB）作为一种良好的过氧化氢还原催化剂，已与非导电聚邻氨基酚（POAP）膜结合以组装葡萄糖生物传感器。与PB修饰的酶生物传感器相比，基于固定在PB修饰电极上的POAP膜中的葡萄糖氧化酶的生物传感器在中性介质中显示出大大提高的稳定性（30天后仍保留78%）。此外，该生物传感器在0.0 V的施加电位下表现出其他良好特性，如相对较低的检测限（0.01 mM）、短响应时间（5秒内）、大电流密度（0.28 mA/cm²）、高灵敏度（24 mAM⁻¹cm⁻²）和良好的抗干扰能力。酶催化反应的表观活化能和表观米氏常数分别为34.2 KJmol⁻¹和10.5 mM。此外，还研究并讨论了温度、测定中使用的施加电位、检测溶液的pH值以及电活性干扰物对传感器安培响应的影响。
