Department of Chemistry, Institute of Analytical Science, Southwest Normal University, Beibei, Chongqing 400715, People's Republic of China.
Talanta. 2003 Mar 1;59(3):571-6. doi: 10.1016/s0039-9140(02)00568-4.
A chemiluminescence (CL) biosensor on a chip coupled to microfluidic system is described in this paper. The CL biosensor measured 25x45x5 mm in dimension, was readily produced in analytical laboratory. Glucose oxidase (GOD) was immobilized onto controlled-pore glass (CPG) via glutaraldehyde activation and packed into a reservoir. The analytical reagents, including luminol and ferricyanide, were electrostatically co-immobilized on an anion-exchange resin. The most characteristic of the biosensor was to introduce the air as the carrier flow in stead of the common solution carrier for the first. The glucose was sensed by the CL reaction between hydrogen peroxide produced from the enzymatic reaction and CL reagents, which were released from the anion-exchange resin. The proposed method has been successfully applied to the determination of glucose in human serum. The linear range of the glucose concentration was 1.1-110 mM and the detection limit was 0.1 mM (3sigma).
本文介绍了一种芯片上的化学发光（CL）生物传感器与微流控系统相结合。该 CL 生物传感器尺寸为 25x45x5mm，可在分析实验室中轻松制备。葡萄糖氧化酶（GOD）通过戊二醛活化固定在控制孔玻璃（CPG）上，并填充到储液器中。分析试剂，包括鲁米诺和铁氰化物，通过静电共固定在阴离子交换树脂上。该生物传感器的最大特点是首次引入空气作为载体流，而不是常见的溶液载体。葡萄糖通过酶反应产生的过氧化氢与从阴离子交换树脂中释放的 CL 试剂之间的 CL 反应进行检测。该方法已成功应用于人血清中葡萄糖的测定。葡萄糖浓度的线性范围为 1.1-110mM，检测限为 0.1mM（3sigma）。
