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基于葡萄糖氧化酶/聚（3,4-乙撑二氧噻吩）：4-磺酸杯[4]芳烃/二硫化钼复合修饰电极的葡萄糖电化学传感

Electrochemical Sensing of Glucose Using Glucose Oxidase/PEDOT:4-Sulfocalix [4]arene/MXene Composite Modified Electrode.

作者信息

Murugan Preethika, Annamalai Jayshree, Atchudan Raji, Govindasamy Mani, Nallaswamy Deepak, Ganapathy Dhanraj, Reshetilov Anatoly, Sundramoorthy Ashok K

机构信息

Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India.

Department of Biotechnology, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India.

出版信息

Micromachines (Basel). 2022 Feb 16;13(2):304. doi: 10.3390/mi13020304.

DOI:10.3390/mi13020304
PMID:35208428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8877456/
Abstract

Glucose is one of the most important monosaccharides found in the food, as a part of more complex structures, which is a primary energy source for the brain and body. Thus, the monitoring of glucose concentration is more important in food and biological samples in order to maintain a healthy lifestyle. Herein, an electrochemical glucose biosensor was fabricated by immobilization of glucose oxidase (GOX) onto poly(3,4-ethylenedioxythiophene):4-sulfocalix [4]arene (PEDOT:SCX)/MXene modified electrode. For this purpose, firstly, PEDOT was synthesized in the presence of SCX (counterion) by the chemical oxidative method. Secondly, MXene (a 2D layered material) was synthesized by using a high-temperature furnace under a nitrogen atmosphere. After that, PEDOT:SCX/MXene (1:1) dispersion was prepared by ultrasonication which was later utilized to prepare PEDOT:SCX/MXene hybrid film. A successful formation of PEDOT:SCX/MXene film was confirmed by HR-SEM, Fourier transform infrared (FT-IR), and Raman spectroscopies. Due to the biocompatibility nature, successful immobilization of GOX was carried out onto chitosan modified PEDOT:SCX/MXene/GCE. Moreover, the electrochemical properties of PEDOT:SCX/MXene/GOX/GCE was studied through cyclic voltammetry and amperometry methods. Interestingly, a stable redox peak of FAD-GOX was observed at a formal potential of -0.435 V on PEDOT:SCX/MXene/GOX/GCE which indicated a direct electron transfer between the enzyme and the electrode surface. PEDOT:SCX/MXene/GOX/GCE also exhibited a linear response against glucose concentrations in the linear range from 0.5 to 8 mM. The effect of pH, sensors reproducibility, and repeatability of the PEDOT:SCX/MXene/GOX/GCE sensor were studied. Finally, this new biosensor was successfully applied to detect glucose in commercial fruit juice sample with satisfactory recovery.

摘要

葡萄糖是食物中发现的最重要的单糖之一，作为更复杂结构的一部分，它是大脑和身体的主要能量来源。因此，为了保持健康的生活方式，监测食物和生物样品中的葡萄糖浓度更为重要。在此，通过将葡萄糖氧化酶（GOX）固定在聚（3,4-乙撑二氧噻吩）：4-磺酸杯[4]芳烃（PEDOT:SCX）/MXene修饰电极上制备了一种电化学葡萄糖生物传感器。为此，首先通过化学氧化法在SCX（抗衡离子）存在下合成PEDOT。其次，在氮气气氛下使用高温炉合成MXene（一种二维层状材料）。之后，通过超声处理制备PEDOT:SCX/MXene（1:1）分散液，随后用于制备PEDOT:SCX/MXene混合膜。通过高分辨率扫描电子显微镜（HR-SEM）、傅里叶变换红外光谱（FT-IR）和拉曼光谱证实了PEDOT:SCX/MXene膜的成功形成。由于其生物相容性，GOX成功固定在壳聚糖修饰的PEDOT:SCX/MXene/玻碳电极（GCE）上。此外，通过循环伏安法和安培法研究了PEDOT:SCX/MXene/GOX/GCE的电化学性质。有趣的是，在PEDOT:SCX/MXene/GOX/GCE上，FAD-GOX的稳定氧化还原峰在形式电位为-0.435 V时被观察到，这表明酶与电极表面之间存在直接电子转移。PEDOT:SCX/MXene/GOX/GCE对葡萄糖浓度在0.5至8 mM的线性范围内也表现出线性响应。研究了pH值、传感器重现性以及PEDOT:SCX/MXene/GOX/GCE传感器的重复性的影响。最后，这种新型生物传感器成功应用于商业果汁样品中葡萄糖的检测，回收率令人满意。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/5834a1065110/micromachines-13-00304-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/eec0c29ac4fb/micromachines-13-00304-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/e9e51d265ed3/micromachines-13-00304-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/36958d33d69d/micromachines-13-00304-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/e720cd4df486/micromachines-13-00304-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/c82d3b1bb408/micromachines-13-00304-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/cba846c4360d/micromachines-13-00304-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/c70afe61e731/micromachines-13-00304-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/5834a1065110/micromachines-13-00304-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/eec0c29ac4fb/micromachines-13-00304-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/e9e51d265ed3/micromachines-13-00304-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/36958d33d69d/micromachines-13-00304-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/e720cd4df486/micromachines-13-00304-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/c82d3b1bb408/micromachines-13-00304-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/cba846c4360d/micromachines-13-00304-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/c70afe61e731/micromachines-13-00304-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18ca/8877456/5834a1065110/micromachines-13-00304-g007.jpg

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