Analytical Spectroscopy and Sensors Group (GEAS), Analytical Chemistry Department, Science Faculty, University of Zaragoza, 50009 Zaragoza, Spain.
Talanta. 2003 Jun 13;60(2-3):415-23. doi: 10.1016/S0039-9140(03)00075-4.
In this paper an enzymatic fluorometric sensor for glucose determination in drinks is presented. The sensor film was obtained by immobilisation of glucose oxidase chemically modified with a fluorescein derivative (GOx-FS) in a polyacrylamide polymer. During the enzymatic reaction the changes in the fluorescence intensity of the GOx-FS are related to the glucose concentration. Working in FIA mode, the optimum conditions found were: 0.7 ml min(-1) flow rate, 300 mul sample injection and pH 6.5. The sensor responds to glucose concentrations ranging from 400 to 2000 mg l(-1), the reproducibility is around 3% and the life-time is at least 3 months (more than 350 measurements). The sensor was applied to direct glucose determination in drinks with good accuracy; interference caused by the filter effect was avoided by the kinetics of the reaction.
本文介绍了一种用于饮料中葡萄糖测定的酶荧光传感器。传感器膜是通过将荧光素衍生物化学修饰的葡萄糖氧化酶（GOx-FS）固定在聚丙烯酰胺聚合物中获得的。在酶反应过程中，GOx-FS 的荧光强度变化与葡萄糖浓度相关。在 FIA 模式下，发现的最佳条件为：流速 0.7ml min(-1)，进样 300mul，pH 值 6.5。传感器对 400 至 2000mg l(-1) 的葡萄糖浓度有响应，重现性约为 3%，寿命至少为 3 个月（超过 350 次测量）。该传感器应用于饮料中葡萄糖的直接测定，具有良好的准确性；通过反应动力学避免了过滤效应引起的干扰。
