Determination of sildenafil citrate (viagra) and its metabolite (UK-103,320) by square-wave and adsorptive stripping square-wave voltammetry. Total determination in biological samples.

The voltammetric behaviour of Sildenafil citrate (SC) and its main metabolite UK-103,320 (UK) was studied by square wave techniques, leading to two methods for its total determination in biological samples (urine and human serum). The application of the square wave (SW), without the adsorptive accumulation, shows a maximum response at -0.950V. The effect of experimental parameters that affect this determination are discussed. The stripping technique, proved to be more sensitive, yielding signals three times larger than those obtained by applying a square wave scan without the previous accumulation. Two calibration graphs to determine total SC and UK concentration were established. Calibration graph in urine sample was linear in the range 4.4x10(-8) to 4.8x10(-7)M by the stripping mode with an accumulation time of 10s. In the same conditions but in serum sample regression line was linear in the range 3.4x10(-8) to 9.7x10(-7)M. The two proposed methods (SW and square-wave adsorptive stripping voltammetry (SWAdSV)) were applied to the total concentration analysis in eight different biological samples by the standard addition method with satisfactory results in the four different serum samples by the SW and in the four urines by SWAdSV.