Wangfuengkanagul N, Siangproh W, Chailapakul O
Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Patumwan, Bangkok 10330, Thailand.
Talanta. 2004 Dec 15;64(5):1183-8. doi: 10.1016/j.talanta.2004.04.032.
A method using flow injection (FI) with amperometric detection at anodized boron-doped diamond (BDD) thin films has been developed and applied for the determination of tetracycline antibiotics (tetracycline, chlortetracycline, oxytetracycline and doxycycline). The electrochemical oxidation of the tetracycline antibiotics was studied at various carbon electrodes including glassy carbon (GC), as-deposited BDD and anodized BDD electrodes using cyclic voltammetry. The anodized BDD electrode exhibited well-defined irreversible cyclic voltammograms for the oxidation of tetracycline antibiotics with the highest current signals compared to the as-deposited BDD and glassy carbon electrodes. Low detection limit of 10nM (signal-to-noise ratio = 3) was achieved for each drug when using flow injection analysis with amperometric detection at anodized BDD electrodes. Linear calibrations were obtained from 0.1 to 50mM for tetracycline and 0.5-50mM for chlortetracycline, oxytetracycline and doxycycline. The proposed method has been successfully applied to determine the tetracycline antibiotics in some drug formulations. The results obtained in percent found (99.50-103.01%) were comparable to dose labeled.
一种采用流动注射（FI）并在阳极氧化硼掺杂金刚石（BDD）薄膜上进行安培检测的方法已被开发出来，并应用于四环素类抗生素（四环素、金霉素、土霉素和强力霉素）的测定。使用循环伏安法在包括玻碳（GC）、沉积态BDD和阳极氧化BDD电极在内的各种碳电极上研究了四环素类抗生素的电化学氧化。与沉积态BDD和玻碳电极相比，阳极氧化BDD电极对四环素类抗生素的氧化呈现出明确的不可逆循环伏安图，且电流信号最高。在阳极氧化BDD电极上采用流动注射安培检测时，每种药物的检测限低至10nM（信噪比=3）。四环素的线性校准范围为0.1至50mM，金霉素、土霉素和强力霉素的线性校准范围为0.5至50mM。所提出的方法已成功应用于测定某些药物制剂中的四环素类抗生素。所得的实测百分比结果（99.50 - 103.01%）与标签剂量相当。
