Hullebusch Eric D van, Utomo Sudarno, Zandvoort Marcel H, L Lens Piet N
Sub-Department of Environmental Technology, Wageningen University, "Biotechnion" - Bomenweg 2, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
Talanta. 2005 Jan 30;65(2):549-58. doi: 10.1016/j.talanta.2004.07.024.
In the last few decades, several sequential extraction procedures have been developed to quantify the chemical status of metals in the solid phase. In this study, three extraction techniques (modified [A. Tessier, P.G.C. Campbell, M. Bisson, Anal. Chem. 51 (1979) 844]; [R.C. Stover, L.E. Sommers, D.J. Silvera, J. Water Pollut. Con. F. 48 (1976) 2165]; and the Bureau Communautaire de Reference (BCR) [K.F. Mossop, C.M. Davidson, Anal. Chim. Acta 478 (2003) 111]) were applied to study the distribution of trace (Co, Ni, Zn and Cu) and major (Mn and Fe) elements in two different anaerobic granular sludges from full-scale methanogenic wastewater treatment plants. The Stover scheme displayed a higher number of fractions that induces a poor recovery compared to the other schemes. The sequential extraction scheme recommended by BCR and the modified Tessier scheme gave similar trends and are sufficiently repeatable and reproducible for application in fractionation studies. However, the BCR scheme seems to be of limited utilisation to study anaerobic matrixes because the extraction stage for its reducible fraction may release substantial amounts of trace elements bound to the organic/sulfides fraction, and consequently, the recovery of trace elements in the oxide fraction may be overestimated at the expense of the oxidisable fraction. As a final conclusion, the modified Tessier scheme seems to be the most suitable scheme to study the metal partitioning in anaerobic granular sludges.
在过去几十年里，已开发出几种连续萃取程序来量化固相金属的化学状态。在本研究中，应用了三种萃取技术（改良的[A. 泰西耶，P.G.C. 坎贝尔，M. 比松，《分析化学》51 (1979) 844]；[R.C. 斯托弗，L.E. 萨默斯，D.J. 西尔韦拉，《水污染控制联邦杂志》48 (1976) 2165]；以及欧盟标准局（BCR）[K.F. 莫索普，C.M. 戴维森，《分析化学学报》478 (2003) 111]）来研究来自全尺寸产甲烷废水处理厂的两种不同厌氧颗粒污泥中痕量（钴、镍、锌和铜）和主要（锰和铁）元素的分布。与其他方案相比，斯托弗方案显示出更多的组分，但回收率较低。BCR推荐的连续萃取方案和改良的泰西耶方案呈现出相似的趋势，并且在分级研究中的应用具有足够的可重复性和再现性。然而，BCR方案在研究厌氧基质方面的应用似乎有限，因为其可还原组分的萃取阶段可能会释放大量与有机/硫化物组分结合的痕量元素，因此，氧化物组分中痕量元素的回收率可能会以可氧化组分为代价被高估。最后得出结论，改良的泰西耶方案似乎是研究厌氧颗粒污泥中金属分配的最合适方案。
