Wang X D, Ar'Rajab A, Ahrén B, Andersson R, Bengmark S
Department of Surgery, Lund University, Sweden.
Transplantation. 1991 Sep;52(3):462-6. doi: 10.1097/00007890-199109000-00013.
Acute liver failure is associated with high mortality. Whether support with transplanted hepatocytes improves the outcome is not established. We studied the potential beneficial effects of intrasplenic transplantation of hepatocytes in conjunction with islets of Langerhans on 90% hepatectomy-induced acute liver failure in rats. We found that all control rats died within 48 hr following 90% hepatectomy. In contrast, the mortality decreased significantly in rats transplanted with 10(7) hepatocytes into the spleen parenchyma at 1-3 days prior to 90% subtotal hepatectomy, whereas no significant reduction in mortality was seen in rats transplanted with hepatocytes immediately after the operation. However, cotransplantation of hepatocytes and 400 isolated pancreatic islets into the spleen reduced mortality when performed immediately after the 90% hepatectomy. Therefore, hepatocyte transplantation reduces mortality after 90% hepatectomy only if performed prior to the hepatectomy. However, transplantation of hepatocytes in conjunction with pancreatic islets reduces mortality when performed at the same time as 90% hepatectomy. Hence, the combined transplantation of hepatocytes and islets might offer support after liver failure.
急性肝衰竭与高死亡率相关。移植肝细胞是否能改善预后尚未明确。我们研究了脾内移植肝细胞联合胰岛对大鼠90%肝切除诱导的急性肝衰竭的潜在有益作用。我们发现，所有对照大鼠在90%肝切除后48小时内死亡。相比之下，在90%肝次全切除术前1 - 3天向脾实质内移植10(7)个肝细胞的大鼠死亡率显著降低，而术后立即移植肝细胞的大鼠死亡率未见显著降低。然而，在90%肝切除术后立即将肝细胞与400个分离的胰岛共同移植到脾脏中可降低死亡率。因此，肝细胞移植仅在肝切除术前进行时才能降低90%肝切除后的死亡率。然而，肝细胞与胰岛联合移植在与90%肝切除同时进行时可降低死亡率。因此，肝细胞和胰岛联合移植可能在肝衰竭后提供支持。
