Qian Lei, Yang Xiurong
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renming Street 5625, Changchun Jilin, China.
Talanta. 2006 Jan 15;68(3):721-7. doi: 10.1016/j.talanta.2005.05.030. Epub 2005 Jul 15.
A new amperometric biosensor for hydrogen peroxide was developed based on cross-linking horseradish peroxidase (HRP) by glutaraldehyde with multiwall carbon nanotubes/chitosan (MWNTs/chitosan) composite film coated on a glassy carbon electrode. MWNTs were firstly dissolved in a chitosan solution. Then the morphology of MWNTs/chitosan composite film was characterized by field-emission scanning electron microscopy. The results showed that MWNTs were well soluble in chitosan and robust films could be formed on the surface. HRP was cross-linked by glutaraldehyde with MWNTs/chitosan film to prepare a hydrogen peroxide biosensor. The enzyme electrode exhibited excellent electrocatalytic activity and rapid response for H(2)O(2) in the absence of a mediator. The linear range of detection towards H(2)O(2) (applied potential: -0.2V) was from 1.67 x 10(-5) to 7.40 x 10(-4)M with correction coefficient of 0.998. The biosensor had good repeatability and stability for the determination of H(2)O(2). There were no interferences from ascorbic acid, glucose, citrate acid and lactic acid.
基于戊二醛交联辣根过氧化物酶（HRP），并在玻碳电极上涂覆多壁碳纳米管/壳聚糖（MWNTs/壳聚糖）复合膜，开发了一种新型的过氧化氢安培生物传感器。首先将多壁碳纳米管溶解在壳聚糖溶液中。然后用场发射扫描电子显微镜对多壁碳纳米管/壳聚糖复合膜的形态进行表征。结果表明，多壁碳纳米管可很好地溶解于壳聚糖中，且能在表面形成坚固的膜。通过戊二醛将辣根过氧化物酶与多壁碳纳米管/壳聚糖膜交联，制备了过氧化氢生物传感器。该酶电极在无媒介体的情况下，对H₂O₂表现出优异的电催化活性和快速响应。对H₂O₂（施加电位：-0.2V）的检测线性范围为1.67×10⁻⁵至7.40×10⁻⁴M，校正系数为0.998。该生物传感器在测定H₂O₂时具有良好的重复性和稳定性。抗坏血酸、葡萄糖、柠檬酸和乳酸均无干扰。
