Huang Ke-Jing, Wang Hong, Guo Yue-Hong, Fan Rong-Li, Zhang Hua-Shan
Department of Chemistry, Wuhan University, Wuhan, China.
Talanta. 2006 Mar 15;69(1):73-8. doi: 10.1016/j.talanta.2005.08.062. Epub 2005 Oct 7.
A new fluorescent probe 1,3,5,7-tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (TMDCDABODIPY) has been developed to detect nitrite in meat products and vegetables. The fluorescence of TMDCDABODIPY is very weak, but when it reacts with nitrite, a strong fluorescent triazole forms in aqueous medium at room temperature, which offers the advantage of specificity and sensitivity for the determination of nitrite. The fluorescence intensity was linear over a nitrite concentration of 9-300 nmol l(-1) with a detection limit of 0.21 nmol l(-1) (S/N=3). The proposed method has been used for the determination of trace nitrite in food products with the recoveries of 94.62-105.48%.
一种新型荧光探针1,3,5,7-四甲基-2,6-二乙氧羰基-8-(3',4'-二氨基苯基)-二氟硼二吡咯(TMDCDABODIPY)已被开发用于检测肉制品和蔬菜中的亚硝酸盐。TMDCDABODIPY的荧光非常微弱，但当它与亚硝酸盐反应时，在室温下的水性介质中会形成一种强荧光三唑，这为亚硝酸盐的测定提供了特异性和灵敏度方面的优势。在亚硝酸盐浓度为9 - 300 nmol l(-1)范围内，荧光强度呈线性关系，检测限为0.21 nmol l(-1)(S/N = 3)。该方法已用于食品中痕量亚硝酸盐的测定，回收率为94.62 - 105.48%。
