Liang Jiangong, Huang Shan, Zeng Danyun, He Zhike, Ji Xinghu, Ai Xinping, Yang Hanxi
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, PR China.
Talanta. 2006 Mar 15;69(1):126-30. doi: 10.1016/j.talanta.2005.09.004. Epub 2005 Oct 20.
Based on the quenching of the fluorescence of CdSe quantum dots (QDs) by spironolactone, a simple, rapid and specific method for spironolactone determination was proposed. In the optimum conditions, spironolactone concentration versus quantum dot fluorescence gave a linear response with an excellent 0.997 correlation coefficient, between 2.5 and 700 mg/mL (6.0-1680 micromol/L) and the limit of detection (S/N=3) was 0.2 microg/mL (0.48 micromol/L). The contents of spironolactone in pharmaceutical tablets were determined by the proposed method and the results agreed with the claimed values. The possible mechanism for the reaction was also discussed.
基于螺内酯对CdSe量子点（QDs）荧光的猝灭作用，提出了一种简单、快速且特异的螺内酯测定方法。在最佳条件下，螺内酯浓度与量子点荧光呈线性响应，相关系数为0.997，线性范围为2.5至700 mg/mL（6.0 - 1680 μmol/L），检测限（S/N = 3）为0.2 μg/mL（0.48 μmol/L）。采用该方法测定了药片中螺内酯的含量，结果与标示值相符。还讨论了该反应可能的机理。
