Zeng Hu-Lie, Li Hai-Fang, Wang Xu, Lin Jin-Ming
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing, China.
Talanta. 2006 Mar 15;69(1):226-31. doi: 10.1016/j.talanta.2005.09.030. Epub 2005 Oct 20.
A beta-cyclodextrin (beta-CD)-bonded gel monolithic column polydimethylsiloxane (PDMS) microfluidic device was developed in a simple and feasible way. Before preparation of gel monolithic column in PDMS microchannel, PDMS surface was activated by UV light to create silanol groups, which is an active molecule to covalently bond 3-(trimethoxysilyl)-propyl methacrylate (Bind-Silane) and seal microfluidic device. By the way, Bind-Silane is a bifunctional molecule to link polyacrylamide (PAA) gel and inner wall of PDMS microchannel covalently. Allyl-beta-CD was used not only as a multifunctional crosslinker in PAA gel to control the size of the pores, but also as a chiral selector for the enantioseparation. The stability, transferring heat and optical characteristic of the microfluidic device were examined. The separation capability of the gel monolithic column was confirmed by the successful separation of fluorescein isothiocyanate (FITC)-labeled arginine (Arg), glutamine acid (Glu), tryptophan (Try), cysteine (Cysteine) and phenylalanine (Phe) in the PDMS microfluidic device less than 100 s at 36 mm effective separation length. A maximum of 2.06 x 10(5) theoretical plates was obtained by the potential strength of 490 V/cm. A pair of FITC-labeled dansyl-D,L-threonine (Dns-Thr) was separated absolutely.
以简单可行的方式开发了一种β-环糊精（β-CD）键合凝胶整体柱聚二甲基硅氧烷（PDMS）微流控装置。在PDMS微通道中制备凝胶整体柱之前，通过紫外光活化PDMS表面以产生硅醇基团，硅醇基团是一种活性分子，可与甲基丙烯酸3-（三甲氧基硅基）丙酯（Bind-Silane）共价键合并密封微流控装置。顺便说一下，Bind-Silane是一种双功能分子，可将聚丙烯酰胺（PAA）凝胶与PDMS微通道内壁共价连接。烯丙基-β-CD不仅用作PAA凝胶中的多功能交联剂以控制孔的大小，还用作对映体分离的手性选择剂。对微流控装置的稳定性、传热和光学特性进行了研究。在36 mm有效分离长度下，不到100 s就在PDMS微流控装置中成功分离了异硫氰酸荧光素（FITC）标记的精氨酸（Arg）、谷氨酸（Glu）、色氨酸（Try）、半胱氨酸（Cysteine）和苯丙氨酸（Phe），从而证实了凝胶整体柱的分离能力。在490 V/cm的电场强度下，获得了最多2.06×10⁵理论塔板数。一对FITC标记的丹磺酰-D，L-苏氨酸（Dns-Thr）被完全分离。
