He Deyong, Zhang Zhujun, Zhou Houjiang, Huang Ying
Institute of Analytical Science, The Key Lab of Analytical Chemistry of Chongqing, Southwest Normal University, Beibei, Chongqing, PR China.
Talanta. 2006 Jul 15;69(5):1215-20. doi: 10.1016/j.talanta.2005.12.043. Epub 2006 Jan 31.
Based on a molecularly imprinted polymer (MIP) as the recognition element, a novel chemiluminescence (CL) micro flow sensor on a chip for the determination of terbutaline in human serum is described. The MIP was prepared by using terbutaline as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, and acetonitrile as the solvent. The chip was fabricated from two 50 mm x 40 mm x 5 mm transparent poly (methylmethacrylate) (PMMA) slices. The microchannels on the chip etched by CO(2) laser were 200 microm wide and 150 microm deep. The microsensor cell filled with 2mg MIP for selectively on line adsorbing terbutaline was 10 mm long, 1 mm wide, and 0.5 mm deep. All reagents were controlled by the syringe pump with an accurate timer. The on line adsorbed terbutaline by the MIP can enhance the CL intensity of the reaction of luminol with ferricyanide. The enhanced CL intensity is linear with terbutaline concentration from 8.0 to 100 ng/mL with a detection limit of 4.0 ng/mL (3sigma). The micro flow sensor provides for good reproducibility with the relative standard deviation of 3.6% (n=7) for 20 ng/mL terbutaline.
基于分子印迹聚合物（MIP）作为识别元件，描述了一种用于测定人血清中特布他林的新型芯片上化学发光（CL）微流传感器。以特布他林为模板，甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联单体，乙腈为溶剂制备MIP。芯片由两片50mm×40mm×5mm的透明聚甲基丙烯酸甲酯（PMMA）片制成。用CO₂激光蚀刻的芯片上的微通道宽200μm，深150μm。填充2mg MIP用于选择性在线吸附特布他林的微传感器池长10mm，宽1mm，深0.5mm。所有试剂由带有精确定时器的注射泵控制。MIP在线吸附的特布他林可增强鲁米诺与铁氰化物反应的CL强度。增强的CL强度与特布他林浓度在8.0至100 ng/mL范围内呈线性关系，检测限为4.0 ng/mL（3σ）。该微流传感器具有良好的重现性，对于20 ng/mL特布他林，相对标准偏差为3.6%（n = 7）。
