Xiong Yan, Zhou Houjiang, Zhang Zhujun, He Deyong, He Chao
Institute of Analytical Science, Department of Chemistry, Southwest China University, Beibei, Chongqing 400715, PR China.
J Pharm Biomed Anal. 2006 Jun 7;41(3):694-700. doi: 10.1016/j.jpba.2006.01.008. Epub 2006 Feb 3.
A novel flow injection chemiluminescence (CL) sensor for hydralazine determination using molecularly imprinted polymer (MIP) as recognition element is reported. Hydralazine-MIP was prepared through non-covalent copolymerization using methacrylic acid (MAA) monomer, hydralazine template and ethylene glycol dimethacrylate (EGDMA) cross-linker. Particles of the MIP were packed into a v-shape glass tube for on-line adsorption of the analyte of hydralazine. The adsorbed hydralazine could be sensed by its great enhancing effect on the CL reaction between luminol and periodate. The CL intensity is linear to hydralazine concentration in the range from 2x10(-9) to 8x10(-7) g/mL. The detection limit is 6x10(-10) g/mL (3sigma) and the relative standard deviation is 2.8% (n=7) for 8x10(-9) g/mL hydralazine. The selective experiment showed that the selectivity and sensitivity of the CL method could be greatly improved when MIP was used as recognition element in the flow-injection CL sensor. The sensor was reversible and reusable. It could be used for more than 100 times. It has been used directly to determine the hydralazine in human urine.
报道了一种新型流动注射化学发光（CL）传感器，用于以分子印迹聚合物（MIP）作为识别元件测定肼屈嗪。通过使用甲基丙烯酸（MAA）单体、肼屈嗪模板和乙二醇二甲基丙烯酸酯（EGDMA）交联剂进行非共价共聚制备了肼屈嗪-MIP。将MIP颗粒填充到V形玻璃管中，用于在线吸附肼屈嗪分析物。吸附的肼屈嗪可通过其对鲁米诺与高碘酸盐之间CL反应的显著增强作用来检测。CL强度与肼屈嗪浓度在2×10⁻⁹至8×10⁻⁷ g/mL范围内呈线性关系。检测限为6×10⁻¹⁰ g/mL（3σ），对于8×10⁻⁹ g/mL肼屈嗪，相对标准偏差为2.8%（n = 7）。选择性实验表明，当在流动注射CL传感器中使用MIP作为识别元件时，CL方法的选择性和灵敏度可大大提高。该传感器具有可逆性和可重复使用性。它可以使用100多次。已直接用于测定人尿中的肼屈嗪。
