用于测定海水中生化需氧量的光学生物传感器。

Optical biosensor for the determination of BOD in seawater.

作者信息

Jiang Yaqi, Xiao Lai-Long, Zhao Li, Chen Xi, Wang Xiaoru, Wong Kwok-Yin

机构信息

Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

出版信息

Talanta. 2006 Aug 15;70(1):97-103. doi: 10.1016/j.talanta.2005.11.046. Epub 2006 Feb 14.

DOI:10.1016/j.talanta.2005.11.046

PMID:18970735

Abstract

An automatic sensing system was developed using an optical BOD sensing film. The sensing film consists of an organically modified silicate (ORMOSIL) film embedded with an oxygen-sensitive Ru complex. A multi-microorganisms immobilization method was developed for the BOD sensing film preparation. Three different kinds of microorganisms, Bacillus licheniformis, Dietzia maris and Marinobacter marinus from seawater, were immobilized on a polyvinyl alcohol ORMOSILs. After preconditioning, the BOD biosensor could steadily perform well up to 10 months. The linear fluctuant coefficients (R(2)) in the range of 0.3-40mgL(-1) was 0.985 when a glucose/glutamate BOD standard was applied. The reproducible response for the BOD sensing film could be obtained within +/-2.3% of the mean value in a series of 10 samples in 5.0mgL(-1) BOD standard GGA solution. The effects of temperature, pH and sodium chloride concentration on the two microbial films were studied as well. The BOD sensing system was tested and applied for the BOD determination of seawater.

摘要

利用光学生化需氧量（BOD）传感膜开发了一种自动传感系统。该传感膜由嵌入氧敏钌配合物的有机改性硅酸盐（ORMOSIL）膜组成。开发了一种用于制备BOD传感膜的多微生物固定化方法。将三种不同的微生物，即地衣芽孢杆菌、海Dietzia菌和海海杆菌，固定在聚乙烯醇ORMOSILs上。经过预处理后，BOD生物传感器在长达10个月的时间内都能稳定良好地运行。当应用葡萄糖/谷氨酸BOD标准时，在0.3 - 40mgL(-1)范围内的线性波动系数（R(2)）为0.985。在5.0mgL(-1) BOD标准GGA溶液中，一系列10个样品的BOD传感膜的可重复响应在平均值的+/-2.3%范围内。还研究了温度、pH值和氯化钠浓度对两种微生物膜的影响。对BOD传感系统进行了测试，并将其应用于海水的BOD测定。