Feng Ke-Jun, Yang Yun-Hui, Wang Zhi-Jie, Jiang Jian-Hui, Shen Guo-Li, Yu Ru-Qin
State Key Laboratory for Chemo/biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Talanta. 2006 Oct 15;70(3):561-5. doi: 10.1016/j.talanta.2006.01.009. Epub 2006 Feb 17.
CeO(2)/Chitosan (CHIT) composite matrix was firstly developed for the single-stranded DNA (ssDNA) probe immobilization and the fabrication of DNA biosensor related to the colorectal cancer gene. Such matrix combined the advantages of CeO(2) and chitosan, with good biocompatibility, nontoxicity and excellent electronic conductivity, showing the enhanced loading of ssDNA probe on the surface of electrode. The preparation method is quite simple and inexpensive. The hybridization detection was accomplished by using methylene blue (MB), an electroactive lable, as the indicator. The differential pulse voltammetry (DPV) was employed to record the signal response of MB and determine the amount of colorectal cancer target DNA sequence. The experimental conditions were optimized. The established biosensor has high detection sensitivity, a relatively wide linear range from 1.59x10(-11) to 1.16x10(-7)molL(-1) and the ability to discriminate completely complementary target sequence and four-base-mismatched sequence.
二氧化铈（CeO₂）/壳聚糖（CHIT）复合基质首次被用于固定单链DNA（ssDNA）探针，并用于制备与结直肠癌基因相关的DNA生物传感器。这种基质结合了CeO₂和壳聚糖的优点，具有良好的生物相容性、无毒性和优异的电子导电性，显示出增强的ssDNA探针在电极表面的负载量。制备方法相当简单且成本低廉。杂交检测通过使用电活性标记物亚甲基蓝（MB）作为指示剂来完成。采用差分脉冲伏安法（DPV）记录MB的信号响应并确定结直肠癌靶DNA序列的量。对实验条件进行了优化。所建立的生物传感器具有高检测灵敏度，线性范围相对较宽，从1.59×10⁻¹¹到1.16×10⁻⁷molL⁻¹，并且能够区分完全互补的靶序列和四碱基错配序列。
