Sutherland I A, Mounsey A, Holmes P H
Department of Veterinary Physiology, University of Glasgow Veterinary School, UK.
Vet Parasitol. 1991 Jul;39(1-2):13-7. doi: 10.1016/0304-4017(91)90057-3.
Isometamidium chloride (Samorin, RMB, England) is a widely used and highly effective trypanocide for the treatment of bovine trypanosomiases. However, the appearance of isometamidium-resistant populations of T. congolense in Africa makes it necessary to develop methods for the rapid and reliable detection of drug resistance in the laboratory. Currently available tests are time-consuming and/or expensive. In the present study, the short-term in vitro incubation of trypanosomes in a range of isometamidium concentrations and the infectivity of the parasites in mice has been assessed. A series of T. congolense isolates were used which were known to differ in their in vivo sensitivity to the drug. The results showed a close correlation between the known level of resistance and the capability of trypanosomes to remain infective after incubation in isometamidium. Thus isolates displaying a high level of resistance in vivo remained infective following incubation in higher concentrations of drug. This assay may provide a simple and reliable method for detecting drug resistance in T. congolense.
氯异美啶（Samorin，RMB，英国）是一种广泛使用且高效的锥虫杀灭剂，用于治疗牛锥虫病。然而，非洲出现了对氯异美啶耐药的刚果锥虫种群，这使得有必要开发在实验室中快速可靠地检测耐药性的方法。目前可用的检测方法既耗时又昂贵。在本研究中，评估了锥虫在一系列氯异美啶浓度下的短期体外培养以及这些寄生虫在小鼠体内的感染性。使用了一系列已知在体内对该药物敏感性不同的刚果锥虫分离株。结果表明，已知的耐药水平与锥虫在氯异美啶中孵育后保持感染性的能力密切相关。因此，在体内显示出高耐药水平的分离株在较高浓度药物孵育后仍具有感染性。该检测方法可能为检测刚果锥虫的耐药性提供一种简单可靠的方法。
