Rueff-Barroso Carlos Romualdo, Milagres Débora, do Valle Juliana, Casimiro-Lopes Gustavo, Nogueira-Neto José Firmino, Zanier José Fernando Cardona, Porto Luis Cristóvão
Tissue Repair Laboratory, Department of Histology and Embryology, Rio de Janeiro State University, Brazil.
Med Sci Monit. 2008 Nov;14(11):BR231-6.
To investigate the effects of low-intensity exercise on bone healing during a short time.
MATERIAL/METHODS: We made a surgical 1-mm perforation in the upper third medial cortical of the right tibia of 45 male Wistar rats (3 months old; mean weight, 282+/-34 g). Animals were randomly assigned to a swimming exercise group (SWIM, n=15), a running exercise group (RUN, n=15), or a no exercise control group (CON, n=15). Treatment sessions (10 minutes/day, 5 days/week) were done for 7, 14, or 21 days. Tibias were removed for radiographic, morphometric, and stereologic analyses. Blood samples were obtained for biochemical analyses.
Serum phosphorus levels were higher in animals in the RUN group compared with animals in the SWIM group on the seventh day. On the 14th day, the tibias of the animals in the SWIM and RUN groups exhibited higher radiopacity in radiographic grades than animals in the CON group. No difference in collagen morphometry was verified. On the 21st day, serum alkaline phosphatase levels were higher in animals in the CON group than they were in the exercise groups, and animals in the SWIM and CON groups demonstrated an increase in newly formed bone in comparison to animals in the RUN group.
At the 14th day of treatment, weight-bearing exercise, assessed by radiography, was found to be beneficial for bone healing. Results at the 21st day of treatment further supported the benefits of non-weight-bearing exercises, showing that weight-bearing exercise may improve bone repair in rats.
研究短时间内低强度运动对骨愈合的影响。
材料/方法：对45只雄性Wistar大鼠（3个月大；平均体重282±34克）的右胫骨上三分之一内侧皮质进行1毫米的手术穿孔。将动物随机分为游泳运动组（SWIM，n = 15）、跑步运动组（RUN，n = 15）或无运动对照组（CON，n = 15）。治疗疗程（每天10分钟，每周5天）持续7、14或21天。取出胫骨进行影像学、形态学和体视学分析。采集血样进行生化分析。
在第7天，RUN组动物的血清磷水平高于SWIM组动物。在第14天，SWIM组和RUN组动物的胫骨在影像学分级上的不透射线性高于CON组动物。胶原形态学未发现差异。在第21天，CON组动物的血清碱性磷酸酶水平高于运动组，并且SWIM组和CON组动物与RUN组动物相比，新形成的骨有所增加。
在治疗第14天，通过影像学评估发现负重运动有利于骨愈合。治疗第21天的结果进一步支持了非负重运动的益处，表明负重运动可能改善大鼠的骨修复。
