White Martin A, Eykelenboom John K, Lopez-Vernaza Manuel A, Wilson Emily, Leach David R F
Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Nature. 2008 Oct 30;455(7217):1248-50. doi: 10.1038/nature07282.
It has long been known that the 5' to 3' polarity of DNA synthesis results in both a leading and lagging strand at all replication forks. Until now, however, there has been no evidence that leading or lagging strands are spatially organized in any way within a cell. Here we show that chromosome segregation in Escherichia coli is not random but is driven in a manner that results in the leading and lagging strands being addressed to particular cellular destinations. These destinations are consistent with the known patterns of chromosome segregation. Our work demonstrates a new level of organization relating to the replication and segregation of the E. coli chromosome.
长期以来，人们都知道DNA合成的5'到3'极性在所有复制叉处都会产生一条前导链和一条后随链。然而，直到现在，还没有证据表明前导链或后随链在细胞内以任何方式进行空间组织。在这里，我们表明大肠杆菌中的染色体分离并非随机的，而是以一种导致前导链和后随链被导向特定细胞目的地的方式驱动的。这些目的地与已知的染色体分离模式一致。我们的工作证明了与大肠杆菌染色体复制和分离相关的一种新的组织水平。
